蘆薈多酚配醣體之定量   全文下載

Determination of Marked Components —aloin and aloe-emodin— in Aloe vera before and after hydrolysis

本研究探討蘆薈萃取物之最佳水解條件，並以高效液相層析之梯度沖提方法分析蘆薈萃取物及其水解物之主成分含量。中藥所含之多酚類成分主要以配醣體之形式存在，而許多配醣體需經過腸道內之酵素或菌叢水解成極性較低之?元，方可被人體吸收，因此在品管上若儘量能掌握各具活性?元之總配醣體含量，則更貼近實際可為人體吸收之量，以再提升中藥之品質管制。本研究主要對象為經常應用於中藥及化妝品之蘆薈，定量其水解前後蘆薈大黃素及蘆薈素之含量。結果顯示，本分析方法具良好之線性關係（r > 0.9997），其檢量線確效（< 10%）良好，蘆薈水煎劑之aloin之含量在水解後分別為76.1 ± 5.9 μmol/g及77.7 ± 2.8 μmol/g，而aloe-emodin之含量在水解後分別為8.3 ± 0.5 μmol/g及8.1 ± 0.3 μmol/g，顯示蘆薈之aloin及aloe-emodin含量在水解前後無明顯差異，主因中藥房所購得之蘆薈已經炮製，因此其配醣體已轉化為糖?。本研究建立之方法，可應用於蘆薈之總配醣體含量測定及品質管制。

This article proposed an optimal hydrolysis condition and a chromatographic method for the analysis of polyphenol glycosides in aloe extract and its hydrolysates. The aglycones assayed were aloin and aloe-emodin. The linearity (r > 0.9997) and validation (< 10%) of the calibration curves were excellent. The amounts of aloin were 76.1 ± 5.9 and 77.7 ± 2.8 μmol/g in decoction and hydrolysate, respectively, while those of aloe-emodin were 8.3 ± 0.5 and 8.1 ± 0.3 μmol/g. The contents of aloin and aloe-emodin in aloe showed no significant difference before and after hydrolysis. This may be due to that aloe sold in Chinese medicine retailer was processed and the glycosides have already been transferred to aglycones. In addition, the components may be decomposed during processing. The method developed here can be applied to determine aglycones and their glycosides in aloe for the quality control.