膽酸（Cholic acid）和膽醇鹽（Cyprinol sulfate）對鏈球菌抗生素（Streptozocin）誘發大鼠之毒性並無改善作用   全文下載

Ineffectiveness of Cholic Acid and Cyprinol Sulfate on the Toxicity of Streptozotocin in Rats

有些華人認為攝食動物的膽汁可以改善視力功能，為研究膽酸和膽醇對視力的影響，以鏈球菌抗生素（Streptozocin，STZ）誘發Wistar大鼠產生高血糖大鼠，將48隻大鼠分成兩大組，分別為無STZ組和STZ處理組。無STZ組大鼠於試驗前4天，每天各腹腔注射生理食?水0.6 mL；STZ組大鼠，在試驗第1天，以腹腔各注射STZ（60 mg/kg溶於0.6 mL生理食鹽水並含有10 mM檸檬酸鹽pH 4.5）後，第4天經檢查具有糖尿病大鼠，其後各大組再分4群，每組各6隻，每隔3天各組大鼠分別以經口胃管餵予生理食鹽水1組（saline），膽酸2組（37 mg/kg cholic acid和74 mg/kg cholic acid溶於0.6 mL生理食鹽水）和膽醇鹽1組（37 mg/kg cyprinol sulfate溶於0.6 mL生理食鹽水）共29天。經實驗結果發現各組大鼠肝臟和腎臟對體重之相對比值，血液中紅血球濃度、血紅蛋白和血容積比，血漿中氨基丙胺酸轉胺?（ALT）、氨基麩草醋酸轉胺脢（AST）和鹼性磷酸?（ALP）活性，及尿素氮（BUN）和肌酸酐（Creatinine）等皆無顯著差異。但STZ處理組血漿中葡萄糖和肝臟氧化產物TBARS值均較STZ未處理組者為高，眼球維生素A反而降低。經37和74 mg/kg膽酸和37 mg/kg 膽醇鹽處理後，對STZ引起之3項指標血漿中葡萄糖、肝臟氧化產物TBARS值和維生素A均無顯著差異，顯示膽酸和膽醇鹽對STZ引起之毒性並無改善作用。

The possible effects of bile acid and bile alcohol on the visual acuity of streptozotocin (STZ)-induced hyperglycemia male Wistar rats were investigated. Forty-eight rats were divided into two groups, STZ and non-STZ, and intraperitoneally injected with STZ (60 mg/kg body weight in 0.6 mL of saline) and saline (0.6 mL) on day 1, respectively. After 4 days of STZ treatment, all the rats were tested for glycosuria and then divided into four groups. Each group had 6 rats and the rats were treated orally every 3 days with saline and the non-toxic dosages of cholic acid (37 mg/kg) and cholic acid (74 mg/kg) combined with cyprinol sulfate (37 mg/kg). At the end of the 29-day experiment, the results revealed that the relative ratios of liver and kidney weight to body weight, the concentrations of RBC, hemoglobin and hematocrit in the blood, as well as the levels of AST, ALT, ALP, BUN and creatinine in the plasma of the rats were not significantly different. However, the levels of plasma glucose and liver thiobarbituric acid-relative substances (TBARS) in the rats treated with STZ were higher, while the levels of vitamin A in the cornea were contrarily lower than those of the non-STZ rats. The values of three indicators (plasma glucose, liver TBARS and vitamin A) related to visual acuity were not significantly influenced when the rats were treated with cholic acid alone or combined with cyprinol sulfate in the STZ or non-STZ group. The results indicated that both cholic acid and cyprinol sulfate could not influence the toxicity of STZ in rats.