女貞子水萃取物的鎮痛與抗發炎作用   全文下載

Analgesic and Anti-Inflammatory Activities of Aqueous Extracts of Fructus Ligustri Lucidi

女貞子在中國傳統醫藥被使用於抗發炎。本研究探討女貞子水萃取物（AFLL）在小鼠的鎮痛和抗發炎作用活性機制。在不同試驗模式下， AFLL顯著抑制自由基和脂質過氧化產生。AFLL所具有的化合物包括槲皮素、楊梅素、咖啡酸、沒食子酸和鞣花酸可以清除自由基和在巨噬細胞降低LPS誘導的一氧化氮（NO）產生。AFLL也具有濃度相關性抑制醋酸所引發小鼠扭體反應和福馬林所引發的致痛作用，與卡拉膠在小鼠後足蹠所引發的腫脹反應。AFLL的抗發炎作用，可能藉由抑制一氧化氮和腫瘤壞死因子，並增加抗氧化酵素過氧化氫?（CAT），超氧化物歧化?（SOD），穀胱甘?過氧化?（GPX）活性。利用西方墨點法分析，發現AFLL也減少卡拉膠誘導的誘導型一氧化氮合成?（iNOS）和環氧合成?-2（COX-2）蛋白表現量。這些結果表明，AFLL的抗發炎機制可能藉由增加CAT， SOD和GPX的活性，進一步減少MDA，iNOS，COX-2的表現。由本次試驗結果表明，AFLL可以作為抗氧化作用、鎮痛、抗發炎作用的天然來源。

Fructus Ligustri Lucidi has been used for its anti-inflammatory effects in traditional Chinese medicine. This study investigated the analgesic and anti-inflammatory effects of aqueous extracts of Fructus Ligustri Lucidi (AFLL) in mice. AFLL significantly inhibited the production of radicals and lipid oxidation in various models. The reference compounds present in AFLL, including quercetin, myricetin, caffeic acid, gallic acid and ellagic acid, exhibited the pharmacological activities by scavenging radicals and decreasing LPS-induced nitric oxide (NO) production in macrophages. Administration of AFLL showed a concentration dependent inhibition on the number of acetic acid-induced writhing responses and formalin-induced pain in the late phase, and paw edema development after Carr treatment in mice. The anti-inflammatory activities of AFLL could be via NO and tumor necrosis factor α (TNF-α) suppression and associated with the increase in the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). Western blotting revealed that AFLL decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. These results suggest that anti-inflammatory mechanisms of AFLL might be correlated to the decrease in the level of malondialdehyde (MDA), iNOS and COX-2 in the edema paw via increasing the activities of CAT, SOD and GPx in the liver. Overall, the results showed that AFLL could serve as a natural source of antioxidant, analgesic and anti-inflammation.