以聚伊文思藍修飾電極測定茶及藥品製劑中的蘆丁和葛根素   全文下載

Determination of Rutin and Puerarin in Teas and Pharmaceutical Preparations Using Poly (Evans Blue) Film-Modified Electrodes

以製備的單壁碳奈米管／聚伊文思藍修飾玻璃碳電極，採用循環伏安法，成功建立了一種同時檢測蘆丁和葛根素含量的電化學分析法。結果顯示，與裸玻碳電極相比，單壁碳奈米管／聚伊文思藍修飾電極能顯著提高蘆丁和葛根素的峰電流信號。本電極對於蘆丁和葛根素氧化有優異的電化學活性，在優化的實驗條件下，蘆丁和葛根素分別在510及810mV有良好氧化峰的反應，電流與濃度分別在1.6 × 10-7-2.0 × 10-5 mol/L和3.0 × 10-7-4.6 × 10-5 mol/L範圍內呈現良好的線性關係，蘆丁和葛根素的檢測極限分別為8.2 × 10-8 mol/L和1.2 × 10-7 mol/L。此電極成功應用於測定茶葉和藥品製劑中的蘆丁和葛根素的含量。

Single-walled carbon nanotubes (SWCNTs)/poly (Evans blue)(poly-EB) film-modified glassy carbon (GC) electrodes were fabricated for the voltammetric determination of rutin and puerarin in teas and pharmaceutical preparations. The SWCNTs/poly-EB film-modified electrode showed excellent electrocatalytic activity towards the oxidation of rutin and puerarin. Two well-defined oxidation peaks of rutin and puerarin appeared at 510 and 810 mV, respectively. The peak current depended linearly on the concentration of rutin and puerarin in the range from 1.6 × 10-7 to 2.0 × 10-5 mol/L and 3.0 × 10-7 to 4.6 × 10-5 mol/L, respectively, with the detection limit of 8.2 × 10-8 mol/L for rutin and 1.2 × 10-7 mol/L for puerarin. The modified electrode was successfully applied to the determination of rutin and puerarin in teas and pharmaceutical preparations.