微胞液相層析法同時分析灌溉水中保米黴素及嘉賜黴素   全文下載

Simultaneous Analysis of Blasticidin S and Kasugamycin with Micellar Liquid Chromatography

本研究建立一個簡單的微胞逆相高效液相層析法，以同時分析殺菌劑保米黴素（blasticidin S）和嘉賜黴素（kasugamycin）。層析移動相組成為去離子水：69.3 mM十二烷基硫酸鈉（SDS）（50：1 v/w），流速為1.0 mL/min。層析管柱為Phenomenex(R)Aqua 5μ C18 125A，使用光二極體陣列偵檢器，檢測波長為210 nm，注射體積為20 μL。保米黴素和嘉賜黴素的線性相關係數（r）分別為0.999，0.997。保米黴素和嘉賜黴素的偵測極限（LODs）分別為0.5 μg/mL與0.75 μg/mL，定量極限分別為1.5 μg/mL與2.2 μg/mL。回收率分別為94.9 - 97.3%，87.8 - 99.7%。微胞液相層析法可在10分鐘內同時分析保米黴素（tr = 2.47 min）及嘉賜黴素（tr = 7.15 min）。因此是一具應用價值的微胞液相層析。

A rapid, simple and inexpensive flow injection potentiometric method for the determination of nitrate in leafy vegetables is described. Six commonly consumed fresh leafy vegetables (mint, lettuce, savoy cabbage, stinging nettle, parsley and cress) were obtained from bazaar in Samsun. An all-solid-state miniaturized nitrate-selective electrode was used as a detector in a low-dead-volume flow through cell. Nitrate was determined in the linear range from 0.1 to 100 mmol/L with detection limit of 0.066 mmol/L. The calibration slope is 52.96 ± 1.02 mV/decade in the linear range. The relative standard deviation (RSD) of the electrode response of 100, 10 and 1 mmol/L NO3- was 0.46, 0.49 and 0.98%, respectively. The flow injection analysis (FIA) throughput was 90 samples/h. The FIA results were compared with those obtained by spectrophotometric method. The results of the study show that nitrate contents in fresh leafy vegetables ranged from 97 to 1861 mg/kg.