流動注入電位偵測法檢測葉菜中硝酸塩   全文下載

Determination of Nitrate in Leafy Vegetables by Flow Injection Analysis with Potentiometric Detection

本研究建立快速、簡單且價廉的流動注入電位偵測法檢驗葉菜中硝酸?。 6種常見新鮮葉菜：薄荷、萵苣、甘藍、大蕁麻、荷蘭芹和水芹購自土耳其Samsun區市集。低容量電流通過電池時，全固態微型硝酸?感測電極可做為硝酸?偵測器。硝酸?測定之線性範圍為0.1至100 mmol/L，偵測極限為0.066 mmol/L。線性範圍內之校準斜率為52.96 ± 1.02 mV/decade。硝酸?電極反應100、10及1 mmol/L時，相對標準差分別為0.46、0.49及0.98%。流動注入分析法(FIA)的產能是每小時90件檢體，FIA的檢驗結果並與分光光度法檢驗結果比對，本研究顯示新鮮葉菜檢體中硝酸?含量介於 97至1861 mg/kg間。

A simple and fast micellar liquid chromatographic (MLC) method for simultaneous analysis of the antibiotic fungicides of blasti-cidin S and kasugamycin was developed. Chromatographic separation was achieved on a Phenomenex(R) Aqua 5μ C18 125 A column. The mobile phase consisted of an aqueous mixture of 69.3 mM sodium dodecyl sulfate (SDS) with deionized water (50 : 1, v/w). The flow rate was set at 1.0 mL/min, and the absorbance was monitored at 210 nm with a photodiode array detector. The total run time was less than10 min, and the retention times (tr) were 2.47 min for blasticidin S ion, and 7.15 min for kasugamycin ion. Good linear relationships were obtained with correlation of coefficients (r) of 0.997 for kasugamycin, and 0.999 for blasticidin S. The limits of detection (LODs) of blasticidin S and kasugamycin were 0.5 µg/mL and 0.75 µg/mL, respectively, and the limits of quantitation (LOQs) for blasticidin S and kasugamycin were 1.5 µg/mL and 2.2 µg/mL, respectively. The recoveries of blasticidin S and kasugamycin in blank irrigation water ranged from 94.9 - 97.3% and 87.8 - 99.7%, respectively. By using MLC method blasticidin S and kasugamycin could be analyzed simultaneously.