牛初乳蛋白水解物經由直接模式對 U937 細胞生長之抑制作用   全文下載

Inhibitory Effects of Bovine Colostrum Protein Hydrolysates on Human Leukemic U937 Cell Growth

本 研 究 以 不 同 酵 素（alcalase, flavourzyme, porcine small-intestinal enzymes）進行乳牛分娩後第1至5天之脫脂乳、酪蛋白及乳清之水解反應，並探討所得水解物在濃度100 - 1000 μg/mL 、37°C及5% CO2下作用24 - 120小時對U937細胞生長之抑制作用。結果顯示，初乳蛋白水解物對U937細胞之死亡呈現時間與劑量相關之效應，另外亦發現以豬小腸酵素製備所得之蛋白水解物對U937生長之抑制效應比以其它酵素水解所得者顯著。本研究發現初乳蛋白水解物可抑制U937細胞之生長，而且由脫脂初乳所得者優於由酪蛋白與乳清蛋白所得。"

Skimmed milk, caseins and whey proteins isolated from colostrums collected on the first to fifth days postpartum were hydrolyzed by different enzymes (alcalase, flavourzyme, and porcine small-intestinal enzymes). To investigate how different protein hydrolyastes affect cell death, leukemic U937 cells were treated with different protein hydrolysates at 100 - 1000 μg/mL for 24 to 120 h at 37°C in 5% CO2 incubator. The results showed that protein hydrolysates leading to U937 cell death exhibited dose-and-time-dependent characteris-tics. This study also found that the proteins hydrolyzed by porcine small-intestinal enzymes have more significant effects on cell death than proteins hydrolyzed by other enzymes. In addition, the colostrum protein hydrolysates from skimmed milk were better than those from caseins and whey in inhibiting U937 cell growth.