以體外試驗評估牛蛙（Rana catesbeiana）皮酵素水解物之抗氧化活性及膠原蛋白酶水解物對血管收縮素轉化酶（ACE）的抑制活性   全文下載

In Vitro Evaluation of Antioxidant Activities and Inhibition of ACE Activity by Collagenase-Treated Hydrolysate Derived from Bullfrog Skin

牛蛙皮高溫明膠水解物（retorted skin gelatin hydrolysates, RSGH）經三種酵素（包括膠原蛋白?、?梨蛋白?、胰蛋白?）水解製備，三種酵素水解物的水解率（degree of hydrolysis, DH）均隨水解時間增加而增加。膠原蛋白?水解物（RSGH-Cg）分子量主要分佈於189 - 686 Da（佔40.1 - 50.1%）和686 - 6,511 Da（佔36.7 - 48.7%），其他二種?水解物大多分佈於686 - 6,511 Da（佔58.2 - 60.8%）。所有酵素水解物較初始的高溫明膠水解物具有高效能之抗氧化活性如DPPH自由基清除能力、亞鐵離子螫合能力及還原力。三種酵素水解物中以RSGH-Cg抑制血管收縮素轉化?（angiotensin I-covnerting enzyme, ACE）的IC50值最低，具有最高的抑制ACE活性，並與其水解程度有關。因此，牛蛙皮膠原蛋白?水解物可以成為具有潛力的功能性原料。"

Enzymatic hydrolysates were obtained by hydrolysis of retorted skin gelatin hydrolysates (RSGH) from bullfrog (Rana cates-beiana) using three enzymes including collagenase, bromelain, and pancreatin for various times (0, 15, 30 and 60 min). As the hydro-lysis time increased, degrees of hydrolysis (DH) of all three enzymatic hydrolysates increased. The molecular weight distribution of collagenase-hydrolyzed RSGH (RSGH-Cg) fraction was in the range of 189 - 686 Da (40.1 - 50.1%) and 686 - 6,511 Da (36.7 - 48.7%) while those of the other enzymatic-hydrolyzed RSGH fractions were mostly in the 686 - 6,511 Da (58.2 - 60.8%) range. All enzymatic hydrolysates had more potent antioxidant activity including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferrous ion chelating activity, and reducing power than the original RSGH. RSGH-Cg showed the highest activity. The lower IC50 value of angiotensin I-converting enzyme (ACE) inhibitory activity of RSGH-Cg correlated with the degree of hydrolysis. This study suggested that collagenase treated bullfrog RSGH is a promising functional ingredient.