最適化酵素分解條件以生產具最高脂解促進活性的大豆蛋白水解物   全文下載

Optimization of Enzymatic Hydrolysis Conditions for Producing Soy Protein Hydrolysate with Maximum Lipolysis-Stimulating Activity

利用反應曲面法（response surface methology; RSm）探討2.5%（w/v）分離大豆蛋白（SPi）經1%（w/w of SPi）Flavourzyme(R)水解的最適化條件，所得水解物在成熟的3t3-l1脂肪細胞中可增加丙三醇的釋放量，丙三醇釋放量愈高代表脂解促進活性愈高。影響水解的獨立變數包括水解時間（hydrolysis time; Ht）19.2 - 220.8 min 、pH 5.32 - 8.68 以及反應溫度（reaction temperature; Rt）33.2 - 66.8°C。依據反應曲面和等高線的繪圖結果顯示，Flavourzyme(R)最適化水解SPi的條件為：pH = 7.12, Rt = 48.77°C and Ht = 124.85 min，使其在成熟的3t3-l1脂肪細胞中可得最大的丙三醇釋放量。由於評估缺適性（lack of fit）的 F值並不顯著（p > 0.05），因此以二階（次）模式來描述反應曲面是合宜的。除此之外，此模式有一個滿足的R2 （= 0.935）係數且經實驗所驗證。"

The optimum hydrolysis conditions of 2.5% (w/v) soy protein isolate (SPI) with 1% (w/w of SPI) Flavourzyme(R) for increasing glyc-erol release in mature 3T3-L1 adipocytes were investigated by response surface methodology (RSM). Higher glycerol release indicated higher lipolysis-stimulating activity. The independent variables were hydrolysis time (HT) 19.2 - 220.8 min, pH 5.32 - 8.68 and reaction temperature (RT) 33.2 - 66.8°C. Based on the response surface and contour plots, the optimum hydrolysis of SPI with Flavourzyme(R) for maximizing the glycerol release in the cells occurred at pH = 7.12, RT = 48.77°C and HT = 124.85 min. The F-value for lack of fit was not significant (p > 0.05), so the second order model was appropriate for describing the response surface. In addition, the model had a satisfactory coefficient of R2 (= 0.935) and was verified experimentally.