台灣翻車魨 Masturus lanceolatus 所引起的食物中毒   全文下載

Food Poisoning Caused by Sunfish Masturus lanceolatus in Taiwan

本研究針對在台灣引起食物中毒之翻車魚魚片的毒性和魚種作探討。因有兩人食用翻車魚肉片引起中毒，於是自餐館採集同批冷凍翻車魚魚片，首先進行生物毒性試驗和液相層析儀–質譜儀檢測河魨毒，顯示偵測不到河魨毒。但是殘留的魚肉片經菟葵毒（palytoxin）生物毒性試驗，顯示抽出液對錦鯉具有魚毒性，且對人類、大鼠和吳郭魚的紅血球細胞具有溶血活性。由於中毒患者和老鼠毒性試驗顯現之中毒症狀，具有類似菟葵毒之中毒症狀。因此，這食物之中毒物質推測是由類似菟葵毒化合物所引起的。此外，殘留冷凍翻車魚魚片藉由聚合?鏈鎖反應－－限制片段長度多型性（PCR-RFlP）來鑑定魚種。使用引子（l14735/H15149）來擴增細胞色素b基因部分片段。內切?Stu i被用來進行限制片段長度多型性分析。該可疑致病之魚肉片魚種被鑑定為Masturus lamceolatus。

Attempts was made to clarify the toxicity and species of causative sunfish fillet concerning with a food poisoning. The frozen causative sunfish fillet was collected to determine its toxicity after two people eating it found poisoned. Tetrodotoxin (TTX) was not detected, however, the butanol extract of remained causative muscle showed ichthyotoxicity to gold carp and caused human, rat and tilapia red blood cells haemolysis. Patients and rats showed some symptoms similar to those caused by palytoxin. Hence, it is possible that this food poisoning is caused by a palytoxin-like compound. Moreover, fish species of the sunfish fillet was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A PCR primer set (L14735/H15149) was used to amplify the partial fragment of the cytochrome b gene. Endonucleases Stu I was used in RFLP. The species of the causative sample was identified as Masturus lamceolatus.