運用離子對萃取法（Ion pair Extraction）針對藥物製劑和尿液中氯奎寧（Chloroquine）、乙胺嘧啶（Pyrimethamine）以及甲氧芐啶（Trimethoprim）之分光光度測定   全文下載

Spectrophotometric Determination of Chloroquine, Pyrimethamine and Trimethoprim by Ion Pair Extraction in Pharmaceutical Formulation and Urine

本研究介紹一簡易且準確之測定方法，測定純品、藥物製劑和尿液檢體中之chloroquine diphosphate (CQ), pyrimethamine (PYM) 以及 trimethoprim (TMP) 。本方法應用離子對萃取技術，藉由氯仿及酸鹼指示劑－－溴甲酚紫 (BCP) 作為對應離子加以萃取。因離子對在有機相中呈黃色，故將最大吸收波長（λmax）訂於 420 nm並測量其吸光度。結果顯示，此三項藥品分別在濃度1.25-8.75、0.62-7.5和1.25-10.71 μg/mL 範圍內遵循比爾定律；桑德爾靈敏度（Sandell’s sensitivity）則依序為0.01258、0.01102 和 0.01464 μg/cm2。而Ringbom理想濃度範圍分別位於2.5-7.5、1.25-7.5 以及2.5-10.7 μg/mL，顯出本方法之可信度。同時，該複合物之化學劑量學及熱力學亦透過賈伯法（Job’s method）和Benesi-Hildebrand plot 加以評估。此外，同日間和異日間之估算差異以及與其他方法比較之統計結果也證明本方法具有高度準確性與精準度。總而言之，本方法相當快速、簡易且適合用於品質管控。"

A simple and accurate method for the determination of chloroquine diphosphate (CQ), pyrimethamine (PYM) and trimethoprim (TMP) in pure, pharmaceutical and urine samples is described. The method involved an ion pair extraction procedure with chloro-form using an acid base indicator, bromocresol purple (BCP), as a counter ion. The ion pair extracted in organic phase was yellow, and the absorbance was measured at λmax = 420 nm. Beer’s law was obeyed over the concentration range of 1.25-8.75, 0.62-7.5, and 1.25-10.71 μg/mL, respectively. The Sandell’s sensitivity was 0.01258, 0.01102 and 0.01464 μg/cm2. The Ringbom optimum concentration range, which was 2.5-7.5, 1.25-7.5 and 2.5-10.7 μg/mL for CQ, PYM, and TMP, respectively, exhibited reliability of the method. Stoichiometry and thermodynamic of the complexes were evaluated by Job’s method and Benesi-Hildebrand plot. The statistical results of intra- and inter-day estimation of drugs and comparison with the reported methods demonstrated high precision and accuracy of the method. The procedure was rapid, simple and suitable for quality control purpose.