芝麻木酚素（sesame lignan）顯著緩解四氯化碳添加卡瓦（kava）所導致之大鼠肝損傷   全文下載

Sesame Lignans Significantly Alleviate Liver Damage of Rats Caused by Carbon Tetrachloride in Combination with Kava

本研究主要目的在於探討芝麻木酚素是否可減緩四氯化碳添加卡瓦所導致之大鼠肝損傷。將雄性Sprague-Dawley大鼠分六組（control, Kava, K+S, CCl4, C+K, and C+K+S）分別餵食玉米油當對照組、卡瓦、卡瓦與芝麻木酚素、四氯化碳、四氯化碳與卡瓦、四氯化碳與卡瓦及芝麻木酚素後，持續紀錄其體重變化、飲食攝取量、動物行為模式與血液中丙胺酸轉胺?（alanine aminotransferase, ALT）、天冬胺酸轉胺?（aspartate aminotransfer-ase, AST）及鹼性磷酸?（alkaline phosphatase, ALP）酵素活性。四週後犧牲大鼠，取得器官重量及肝臟脂肪變性、纖維化等組織病理分析數據。第二十八天時，三組攝取四氯化碳之實驗組均表現出飲食攝取量降低及體重減少的現象，同時肝臟重量有明顯增加。因四氯化碳引起之肝臟傷害在合併餵食卡瓦時略有惡化，但若同時餵食芝麻木酚素則肝臟損傷明顯減緩，其結果與肝臟脂肪變性及纖維化評估分級一致。在單獨餵食卡瓦或是餵食卡瓦加芝麻木酚素組動物中，並無任何肝臟損傷現象。此外，血液ALT 、 AST及ALP酵素活性檢測結果同樣顯示，無論是否合併卡瓦萃取物餵食，芝麻木酚素對四氯化碳引起之肝臟損傷有明顯之保護作用。綜此結論，無論是否添加卡瓦，芝麻木酚素均顯著緩解四氯化碳所導致之大鼠肝損傷。同時服用芝麻木酚素可克服卡瓦增加肝損傷之潛在危險，應可增廣卡瓦的應用性。"

The aim of this study was to examine if the liver damage caused by CCl4 in combination with kava (Piper methysticum) could be alleviated via coadministration of sesame lignans. Six groups of male Sprague-Dawley rats were fed with corn oil as a control, kava only, kava plus sesame lignans, CCl4 only, CCl4 plus kava, and CCl4 plus kava plus sesame lignans, respectively. Body weight, diet intake, animal behavior, and serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alka-line phosphatase (ALP) were recorded along with the course. Four weeks later, organ weights and histopathological data including liver steatosis and fibrosis were obtained after sacrifice. At day 28, both the diet intakes and body weights of rats decreased, and their relative liver weights increased considerably in all three CCl4-treated groups compared to the control group. In accord with the levels of pathological steatosis and fibrosis, the liver damage caused by CCl4 was somewhat worse when kava extract was coin-gested, but significantly attenuated when sesame lignans were coadministrated. No detectable liver damage was observed in the kava or kava plus sesame lignan group. The protective effect of sesame lignans against liver injury caused by CCl4 with or without kava extract was also observed in the relative activities of plasma ALT, AST and ALP. It is concluded that sesame lignans signifi-cantly alleviate liver damage of rats caused by carbon tetrachloride with or without kava. The potential risk of kava’s hepatotoxicity can be greatly circumvented and the applications of kava may be further extended by coadministration of sesame lignans.