應用SDS-PAGE 鑑定引起中毒之鯙鰻肌肉的魚種   全文下載

Species Identification of a Causative Moray Eel Meat by SDS-PAGE

本研究嘗試鑑定引起食物中毒之魚種，除了引起食物中毒之鯙鰻魚肉外，另外採集八種常見生鮮鯙鰻，種類包含Gymnothorax favagineus, G. fim-briatus, G. flavimarginatus, G. meleagris, G. pseudothyrsoideus, G. undulates, G. albimarginatus和G. javanicus；檢體以100°C加熱30分鐘後，再以十二烷基磺酸鈉－－聚丙烯醯胺凝膠電泳（SDS-PAGE）加以分析。此八種鯙鰻之SDS-PAGE電泳圖譜顯示有種分子量小於30.0 kD之專一性蛋白帶存在。然後，將利用2% SDS與8 M urea所萃取之蛋白，分別與SDS-PAGE圖譜上小於30.0 kD之部分進行比較，結果顯示，引起中毒之檢體為G. javanicus 。"

Attempts were made to identify the species of food poisoning moray eel. Besides the causative processed moray eel meat, eight other raw commercial moray eel meats including Gymnothorax favagineus, G. fimbriatus, G. flavimarginatus, G. meleagris, G. pseudothyrsoideus, G. undulates, G. albimarginatus and G. javanicus were heated at 100℃ for 30 min and then assayed using the electrophoresis method of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The SDS-PAGE patterns for eight processed commercial moray eel meats showed species-specific protein bands < 30 kD. The species of causative processed moray eel implicated in food poisoning case was identified as G. javanicus in comparison to the 2% SDS and 8 M urea extracted protein revealed by SDS-PAGE patterns.