馬來西亞市售生花生仁黃麴毒素含量調查   全文下載

Aflatoxin in Raw Peanut Kernels Marketed in Malaysia

本研究檢測隨機從馬來西亞超市取得之84件生花生仁中黃麴毒素含量。檢體先經溶劑萃取和免疫親合法淨化（immunoaffinity clean-up）後，為提高檢驗效能，以具管柱後光化學反應器（post-column photochemical reactor）及螢光檢測器之高效液相層析儀檢測黃麴毒素含量。偵測極限和定量極限分別為0.01-0.09及0.04-0.30 ng/mL，檢體中黃麴毒素之濃度範圍從「未檢出」到97.28 ng/g。實驗結果顯示，約78.57%之檢體受到黃麴毒素污染，其中10.71%檢體中黃麴毒素含量更超過食品法典委員會（Codex）所訂之最高容許值（maximum tolerable limit）15 ng/g。濃度0.15 ng/mL時，AFG2和AFB2之平均回收率分別為74.85 ± 8.83%及103.91 ± 6.45%，均在可接受範圍內。本研究估計黃麴毒素平均攝取量為10.69 ng/kg bw/day。檢體中黃麴毒素含量因品牌或產地不同，而有顯著差異（P < 0.05）。

The occurrence of aflatoxin in eighty-four samples of raw peanut kernels which are randomly collected from Malaysian super-markets was examined. Analysis for aflatoxin was performed by solvent extraction and immunoaffinity clean-up followed by the determination using high performance liquid chromatography equipped with post-column photochemical reactor for enhanced detection and fluorescence detector. A detection limit of 0.01-0.09 ng/mL and a quantification limit of 0.04-0.30 ng/mL were obtained. The aflatoxin concentrations ranged from not detected to 97.28 ng/g in all samples investigated. About 78.57% of the samples were contaminated with aflatoxin, of which 10.71% exceeded the maximum tolerable limit of 15 ng/g set by the Codex. Average recoveries of the aflatoxin analysis were acceptable which were in the range of 74.85 ± 8.83% for AFG2 at the concentration of 0.15 ng/mL and 103.91 ± 6.45% for AFB2 at the concentration of 0.15 ng/mL. The average daily intake estimated for total afla-toxins was 10.69 ng/kg body weight. There was a significant difference (P < 0.05) in aflatoxin content between brands and locations.