| 中文摘要 |
本研究探討愛玉子瘦果殘渣水萃物（WerJFA）於自由基傷害DNA時之保護作用，及其對老鼠結腸癌細胞CT-26、人類肝癌細胞Hepg2 和人類乳癌細胞MCF-7 這三種癌細胞株生長之影響。以瓊脂膠體電泳分析（agarose gel electrophoresis）DNA 並以四氮唑鹽 [MTT, 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide] 比色法測定活細胞數目，結果證實WerJFA 具有降低氫氧自由基誘導之DNA 損傷和抑制癌細胞株生長之作用。三種癌細胞株之中以CT-26 之被抑制效果最佳，其次為Hepg2，而MCF-7 細胞之被抑制能力最差；於濃度200 μg WerJFA/mL 下，CT-26 和Hepg2 細胞的生長抑制率分別為85.4% 和60.0%。添加WerJFA 至老鼠肝細胞Clone 9 細胞之培養液中，經MTT 比色法測定，發現WerJFA 在0-200 μg/mL 濃度下並無細胞毒性。由以上結果顯示WerJFA 具有抗癌的活性成分。再經超過濾（ultrafiltration）處理，發現可加強WerJFA 抑制細胞生長的能力，例如濃度200 μg/mL 的3-10 kDa 區分物與Hepg2 細胞共同培養72 hr 後、細胞生長抑制率達到73.5%。" |
| 英文摘要 |
The present study investigated the effect of water extract from the residue of jelly fig achenes (WERJFA) on the protection of DNA against hydroxyl radical damage and on the growth inhibition of three cancer cell lines, including mouse colon cancer CT-26, human hepatoma HepG2 and breast cancer MCF-7. DNA protection against hydroxyl radical from hydrogen peroxide was assayed by agarose gel electrophoresis. Growth inhibition of the cell lines was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that WERJFA was able to reduce the hydroxyl radical-induced DNA damage and to inhibit the growth of the three cancer cell lines with CT-26 being the most vulnerable one, HepG2 the second, while MCF-7 the least affected. The growth inhibition rates of WERJFA (200 μg total solids/mL) on CT-26 and HepG2 were 85.4% and 60.0%, respectively. MTT assay was also applied in the rat hepatocyte Clone 9 cytotoxicity test. No cytotoxicity of WERJFA (0-200 μg total solids/mL) was observed. These lines of evidence support that WERJFA has the potential to be an antitumor agent. Ultrafiltration enhanced the growth inhibitory effect of WERJFA. Among all the fractions of filtrate, the 3-10 kDa fraction had the highest inhibition on HepG2 cells, reaching 73.5% reduction of growth when added at 200 μg total solids/mL and incubated for 72 hr. |
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