利用 SCAR 標記鑑定黃耆類藥材   全文下載

Identification of Astragalus Medicines Using Scar Markers

黃耆為廣泛常用的中藥材， 其正確基原為膜莢黃耆（Astragalus membranaceous）和蒙古黃耆（A. membranaceous var. mongholicus）乾燥的根部，紅耆（Hedysarum polybotrys）則是黃耆的誤用品。利用rAPD 技術進行多型性片段篩選，其中OPB-2 引子對經PCr 反應得到A1（1.3 Kb）片段，OPC-14 引子對得到A2（0.6 Kb）和H1（0.8Kb）兩個片段，分別對膜莢黃耆、蒙古黃耆和紅耆具有物種專一性。為建立黃耆與紅耆的SCAr 分子標記，將A1、A2 與H1 片段進行序列分析，找到對膜莢黃耆具有專一鑑別性的Hg3 和Hg4 引子對；對蒙古黃耆具有專一鑑別性的Hg7 和Hg8 引子對；以及對紅耆具有專一鑑別性的rC3 和rC4 引子對。經與黃耆同屬植物與市售品比對，能成功分辨黃耆藥材之基原。

Astragalus radix is a well-known herbal material in traditional Chinese medicine. Astragalus membranaceous and A. membranaceous var. mongholicus are known as the authentic origins of Astragalus medicines whereas Hedysarum polybotrys is a common adulterant of Astragalus radix in Taiwan. Random amplified polymorphic DNA (RAPD) primers were used to screen the polymorphic fragments. Among them, OPB-2 and OPC-14 directed the amplification of 3 specific fragments A1 (1.3 Kb), A2 (0.6 Kb) and H1 (0.8 Kb) from A. membranaceous, A. membranaceous var. mongholicus and H. polybotrys respectively, which were further converted to sequence characterized amplified region (SCAR) markers. Primer pairs HG3 and HG4 derived from A1, HG7 and HG8 from A2, and RC3 and RC4 from H3, were successfully employed on the authentication of Astragalus medicines and H. polybotrys.