以毛細管膠束電動層析法多重分析製藥中Sulfamethoxazole 及Trimethoprim   全文下載

Optimal Conditions for Extraction and Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Pharmaceuticals by Micellar Electrokinetic Capillary Chromatography

以毛細管膠束電動層析法分析sulfamethoxazole 及trimethoprim，其條件為25° C、30 kV 及15 mbar 壓力，背景電解質為：30 mM 硼酸鹽緩衝溶液（pH 9.0）、60 mM sodium dodecysulfate（SDS）及10%（v/v）乙醇，紫外光波長設定為205 nm。兩種研究成分的標準品混合物經由乙醇/ 去離子水（1:1，v/v）萃取，所得到的回收率是最適化且可接受的。實驗結果顯示，本方法是獨特、精確（sulfamethoxazole 及trimethoprim 的回收率分別為99.9 ± 0.4% 及99.8 ± 0.3%）、線性（相關係數 ? 0.9990）及精確的（RSD低於0.6%）， 可用於錠劑、皮膚用粉末及注射液中sulfamethoxazole 及trimethoprim 之檢測。

A micellar electrokinetic capillary chromatography was performed at 25°C and 30 kV (under pressure of 15 mbar), with 30 mM borate buffer (pH 9.0), 60 mM sodium dodecysulfate, and 10% (v/v) ethanol as background electrolyte for the determination of sulfamethoxazole and trimethoprim. UV detection was at 205 nm. Recoveries were optimal and acceptable after extraction with ethanol / deionized water (1:1, v/v) for both investigated compounds from laboratory mixtures of standards. The method was shown to be specific, accurate (recoveries were 99.9 ± 0.4% for sulfamethoxazole and 99.8 ± 0.3% for trimethoprim), linear over the tested ranges (correlation coefficients ≥ 0.9990) and precise (RSD below 0.6%). The method was applied to determine sulfamethoxazole and trimethoprim in tablets, powder for cutaneous use and solution for infusion.