以萃取分光光度計法檢測純態及製藥配方之威而鋼   全文下載

Extractive Spectrophotometric Determination of Sildenafil Citrate (Viagra) in Pure and Pharmaceutical Formulations

兩種簡單、快速且靈敏的萃取分光光度計法已被開發，用以分析純態及製藥配方之威而鋼。這兩種方法分別依據威而鋼與brilliant blue G（BBG）及溴甲酚紫（BCP）形成氯仿可溶性離子結合物（緩衝溶液及最大吸收波長分別為：pH 1.30 之KCl-HCl 及615 nm ；pH 3.5 之NaOAc- HCl 及406 nm ），反應條件經由最適化以得到最大色彩強度；隨著威而鋼濃度增加，吸收亦呈線性增加，相關係數分別為0.9991 及0.9993。BBG 及BCP 濃度分別在0.1-4.0 及1.0-15 μg/mL 時，系統遵守Beer 定律。各種分析參數於本研究中被評估，實驗結果亦藉由統計數據被確效。普通賦型劑存在的製藥配方並無發現干擾物。

Two simple, rapid and sensitive extractive spectrophotometric methods have been developed for the assay of sildenafil citrate (SC) in pure and pharmaceutical formulations. These methods are based on the formation of chloroform soluble ion-association complexes of SC with brilliant blue G (BBG) and with bromocresol purple (BCP) in KCl-HCl buffer of pH 1.30 (for BBG) and in NaOAc- HCl buffer of pH 3.5 (for BCP) with absorption maximum at 615 nm and 406 nm for BBG and BCP, respectively. Reaction conditions were optimized to obtain the maximum colour intensity. The absorbance was found to increase linearly with increase in concentration of SC, which was corroborated by the calculated correlation coefficient values (0.9991 and 0.9993). The systems obeyed Beer’s law in the range of 0.01-4.0 and 0.021-15.0 μg/mL for BBG and BCP, respectively. Various analytical parameters have been evaluated and the results have been validated by statistical data. No interference was observed from common excipients present in pharmaceutical formulations.