利用芳香取代反應及分光光度計檢測含胺基或硫醇之藥物   全文下載

The Use of an Aromatic Substitution Reaction in the Spectrophotometric Determination of Selected Amino or Thiol Containing Drugs

一項簡單的分光光度計法用以檢測含胺基/ 硫醇之代表藥物acetylcysteine（I）、captopril（II）/ amlodipine besylate（III）、heptaminol hydrochloride（IV）。本方法之原理乃依據這些藥物與活化halide 2,4-dinitrofluorobenzene（DNFB） 在硼酸緩衝溶液中形成黃色產物。（I）、（II）、（III） 及（IV） 的線性（ 遵守Beer 定律） 範圍分別為4-12、2.4-16.8、8-28 及5-25 μg mL-1。不同的實驗參數被探討以求最佳條件。本法係根據USP 27 準則進行確效，適合在無干擾的情況下定量製藥中的相關成分。"

A simple spectrophotometric method is described for the determination of acetylcysteine (I) and captopril (II) as representative examples of thiols , as well as amlodipine besylate (III) and heptaminol hydrochloride (IV) as amine examples. The method is based on the reaction of these drugs with the activated halide 2,4-dinitrofluorobenzene (DNFB) in aqueous borate buffer to yield yellow colored products. Beer’s law was obeyed over the concentration ranges of 4-12, 2.4-16.8, 8-28 and 5-25 μg mL-1 for (I), (II), (III), and (IV), respectively. The different experimental parameters were studied and optimized. The proposed method was validated according to the USP 27 criteria and was found suitable for the quantitation of the cited compounds in their pharmaceutical preparations without interference from common excipients.