補骨脂及市售濃縮製劑中Bakuchiol、Psoralen 及Angelicin 之含量   全文下載

Analysis of Bakuchiol, Psoralen and Angelicin in Crude Drugs and Commercial Concentrated Products of Fructus Psoraleae

利用高效液相層析儀開發補骨脂Psoralea corylifolia L. 中bakuchiol、psoralen 和angelicin 三種指標成分之同時定量分析方法，各指標成分藉由管柱色層分析分離純化並經質譜及核磁共振光譜確認。樣品使用Cosmosil 5C18-aR-ii 管柱，流動相為20% 氰甲烷和氰甲烷之混合溶液採用梯度沖提法，以1.0 mL/min 之流速沖提，偵測波長為254 nm。以積分面積比值計算，同日間（intraday）相對標準偏差在0.3~1.5% 之間，異日間（interday）相對標準偏差在0.2~1.0% 之間。以本實驗開發之 hPLC 分析方法，針對十八種樣品進行定量分析，結果bakuchiol、psoralen 和angelicin 於十種生藥材中之含量範圍分別為36.2~71.0, 2.5~13.0 和2.2~9.2 mg/g；而於八種市售濃縮製劑中之含量範圍則分別為0.6~21.1、0.6~5.2 和0.6~5.3 mg/g。"

The goal of this study was to develop a HPLC method for simultaneously determining bakuchiol, psoralen and angelicin in Fructus Psoraleae (Buguzhi, the fruits of Psoralea corylifolia) and its commercial concentrated products. Extracted samples were analyzed by using a reverse-phase column (Cosmosil 5C18-AR-II) and eluting with a gradient mobile phase consisting of 20% acetonitrile to acetonitrile at a flow rate of 1.0 mL/min with detection at a wavelength of 254 nm. The relative standard deviations of the marker substances on the basis of peak-area ratios in intraday and interday analyses were 0.3~1.5% and 0.2~1.0%, respectively. Bakuchiol, psoralen and angelicin contents were 36.2~71.0, 2.5~13.0 and 2.2~9.2 mg/g for ten raw material samples of Fructus Psoraleae, and 0.6~21.1, 0.6~5.2 and 0.6~5.3 mg/g for eight commercial concentrated products of Fructus Psoraleae, respectively.