台灣野生大豆Glycine dolichocarpa、G. tabacina及G. tomentella異黃酮類成分含量之變異   全文下載

Contents of Certain Isoflavones in Glycine dolichocarpa, G. tabacina and G. tomentella Collected in Taiwan

Glycine tabacina、G. tomentella及G. dolichocarpa為台灣原生之三種多年生野生大豆。台灣金門、澎湖地區以G. tabacina、G. tomentella的根部作為中藥材一條根之基原，使用已有數十年。本研究的目的為開發一適當的高效液相層析分析條件，定量分析此三種野生大豆之異黃酮成分daidzin、daidzein、genistin與genistein含量之差異。三種野生大豆植株各部位之異黃酮總含量都以根部為最高，個別之成分含量除G. tomentella的種子外，各部位皆以daidzein最高。三個種中以G. tabacina根、莖部之異黃酮總含量最高， 葉片的含量以G. tomentella最高， 種子中的含量最高者為G. dolichocarpa，各成分之含量在各部位及種間皆有明顯差異。此結果不僅可作為大豆育種上之參考，也可作為台灣一條根藥材基原鑑定之參考。

Glycine tabacina, G. tomentella and G. dolichocarpa are three wild perennial soybean species native to Taiwan. Glycine tabacina and G. tomentella are the source of I-Tiao-Gung, a Chinese herb. This study was commissioned to measure the presence of isoflavones in each and qualify/quantify isoflavone content using an appropriately configured multi-component HPLC method. Isoflavone in these soybean species was most heavily concentrated in the root systems. Daidzein was the predominant isoflavone found throughout plant structures, with the exception of G. tomentella seeds. Isoflavone concentrations were highest in the roots and stems of G. tabacina, in the leaves of G. tomentella, and in the seeds of G. dolichocarpa. Significant differences in isoflavone levels exist between species and structural components. The results of this study can help set selection and qualification parameters for Glycine germplasm and serve as reference when confirming genetic markers in Chinese herbs.