以高效液相層析法快速測定視網醇、視網醛、13-順式視網酸及全反式視網酸   全文下載

Rapid Measurement of Retinol, Retinal, 13-Cis-Retinoic Acid and All-Trans-Retinoic Acid by High Performance Liquid Chromatography

定組成移動相液相層析法已被發展於快速測定視網醇、視網醛、13-順式視網酸及全反式視網酸。利用85%甲醇及0.01 M醋酸鈉緩衝溶液（pH 5.2）作為移動相，並以流速1.5 mL/min之條件及5 μm C18逆相管柱（4.6 mm I.D., 15 cm）分析上述4種化合物，結果均可在14 min之內沖堤出。維他命A酯亦可用相同條件於25.5 min時沖堤出。本方法可藉由螢光及紫外光檢測器於最適波長下檢測上述各化合物。"

An isocratic liquid chromatographic method has been developed for rapid measurements of retinol, retinal, all-trans-retinoic acid and 13-cis-retinoic acid. Using 85% methanol and 15% 0.01 M sodium acetate buffer, pH 5.2, as the mobile phase and at a flow rate of 1.5 mL/min, retinol, retinal, all-trans-retinoic acid and 13-cis-retinoic acid were eluted from a 5 μm C18 reversed-phase column (4.6 mm I.D., 15 cm) in less than 14 min. Retinyl acetate, when desired, was eluted in 25.5 min under the above conditions. Each compound was detected at the most sensitive wavelength by coupling fluorescence and UV detection.