以DTT保存血漿中總維他命C儲存於4℃之穩定性探討   全文下載

Stability of Total Ascorbic Acid in DTT-Preserved Plasma Stored at 4°C

為了評估總維他命C於4℃冷藏的穩定性，本研究徵求12位志願者，取得其含EDTA的全血並立即加入dithiothreitol（DTT）保存。採血後每位志願者的血液被均分成19份，其中1管立即以毛細管電泳法測定總維他命C的濃度，其餘的小離心管置於4℃冰箱中保存。此後72小時內，每隔4小時拿出1小管檢體以毛細管電泳法測定總維他命C的濃度。結果發現：採血後放置4℃冰箱中16小時內，總維他命C的濃度並無顯著差異（p>0.05）。此後，血液中總維他命C的濃度顯著地降低（p<0.05）。由此實驗結果得知於待測檢體中加DTT置於4℃冰箱中冷藏保存，總維他命C濃度可穩定16小時。因此建議：在臨床檢驗總維他命C濃度時，若無法於抽血後馬上測定，則可加入DTT置於4℃冰箱中冷藏保存，並於16小時內測定，避免檢驗結果之誤差。

In order to evaluate the stability of total ascorbic acid (TAA) after storage in refrigeration at 4˚C, EDTA-blood was collected from 12 volunteers and preserved with dithiothreitol (DTT) immediately. After sampling, the blood was portioned into 19 micro-centrifuge tubes. One tube of sample was examined immediately by the capillary electrophoresis (CE) method, while the others were stored in refrigeration at 4˚C until analysis. TAA analysis were performed once every 4 hr for a total of 72 hr after the sample collection. Our results showed that TAA concentrations in these specimens did not alter significantly within the first 16 hr of storage; however, TAA levels started to decline significantly (p < 0.05) thereafter. These data demonstrated that DTT could be an effective preservative in maintaining the stability of TAA in a refrigerated temperature for up to 16 hr. Thus, it is advised that if the determination of TAA cannot be performed immediately, specimens must be preserved with DTT at the refrigerated temperature and assayed within 16 hr in order to prevent the occurrence of inaccurate results. 為了評估總維他命C於4℃冷藏的穩定性，本研究徵求12位志願者，取得其含EDTA的全血並立即加入dithiothreitol（DTT）保存。採血後每位志願者的血液被均分成19份，其中1管立即以毛細管電泳法測定總維他命C的濃度，其餘的小離心管置於4℃冰箱中保存。此後72小時內，每隔4小時拿出1小管檢體以毛細管電泳法測定總維他命C的濃度。結果發現：採血後放置4℃冰箱中16小時內，總維他命C的濃度並無顯著差異（p>0.05）。此後，血液中總維他命C的濃度顯著地降低（p<0.05）。由此實驗結果得知於待測檢體中加DTT置於4℃冰箱中冷藏保存，總維他命C濃度可穩定16小時。因此建議：在臨床檢驗總維他命C濃度時，若無法於抽血後馬上測定，則可加入DTT置於4℃冰箱中冷藏保存，並於16小時內測定，避免檢驗結果之誤差。