三亞蟎及其代謝產物在尿液中之固相萃取及氣相層析－－質譜儀分析   全文下載

Solid-Phase Extraction and GC-MSD Determination of Amitraz and Metabolites in Urine

三亞?20%乳劑噴施於柑桔及梨園，並於施藥前、後分別收集七位農民之尿液，分析三亞?及其代謝產物BTS-27919, BTS-27271, 2,4-dirnethylaniline之含量。取5 rnL尿液樣品以SPE-C18 （500 mg, 3 mL）淨化，於不同溶劑分段收集三亞?及其代謝產物，並以氣液層析儀附質譜檢測器偵測。本方法回收率三亞?為75.7 ± 4.2 %、BTS-27271為81.3 ± 2.4%、BTS-27919為87.7 ± 3.7%、2,4-dirnethy laniline為83.3 ± 1.8%。而七位農民尿液中均未檢測出三亞?及其代謝產物之殘量。"

Urine samples were collected from seven farmers before and after applying 20% amitraz EC to pear and citrus fields. Amitraz and its metabolites including BTS-27919, BTS-27271, and 2,4-dimethylaniline were eluted with n-hexane, dichloromethane and methanol in sequence from SPE-C18 cartridge by loading 5 mL of urine before GC-MSD determination. The mean recoveries of amitraz, BTS- 27919, BTS-27271 and 2,4-dimethylaniline were 75.7 ± 4.2 , 81.3 ± 2.4 , 87.7 ± 3.7 and 83.3 ± 1.8%, respectively. Neither amitraz nor metabolites were detected in any of the seven farmers’ urine samples. 三亞蟎20%乳劑噴施於柑桔及梨園，並於施藥前、後分別收集七位農民之尿液，分析三亞蟎及其代謝產物BTS-27919, BTS-27271, 2,4-dirnethylaniline之含量。取5 rnL尿液樣品以SPE-C18 （500 mg, 3 mL）淨化，於不同溶劑分段收集三亞蟎及其代謝產物，並以氣液層析儀附質譜檢測器偵測。本方法回收率三亞蟎為75.7 ± 4.2 %、BTS-27271為81.3 ± 2.4%、BTS-27919為87.7 ± 3.7%、2,4-dirnethy laniline為83.3 ± 1.8%。而七位農民尿液中均未檢測出三亞蟎及其代謝產物之殘量。