以液態層析法測定人體血液中的主要類胡蘿蔔素   全文下載

Determination of Major Carotenoids in Human Serum by Liquid Chromatography

一種簡單快速的高效率液相層析法已被發展出，以測定人體血液中的主要類胡蘿蔔素。使用C18管柱配合等比動相甲醇／二氯甲烷（95:5, v／v）及樣品溶劑甲醇／二氯甲烷（45:55, v／v），偵測波長476nm和流速0.8 mL／min，可於20分鐘內分離出葉黃素、β-胡蘿蔔素及番茄紅素，且回收率分別為92, 90及87% 。使用此方法分析由老人安養中心收集的62個血液樣品，結果顯示大部分血液樣品具有最高量的β-胡蘿蔔素，次為葉黃素及番茄紅素。"

A simple and rapid high performance liquid chromatographic (HPLC) method was developed to determine the major carotenoids in human serum. Lutein, β-carotene and lycopene were separated within 20 min using a C18 column and a mobile phase of methanol/methylene chloride (95:5, v/v) with sample solvent methanol/methylene chloride (45:55, v/v), detection at 476 nm and flow rate at 0.8 mL/min. A high recovery was achieved with this method, which amounted to 92, 90 and 87% for lutein, β-carotene and lycopene, respectively, while the detection limit was 5.0, 10.0 and 4.0 ng/mL. This method was applied to determine the major carotenoids in human serum samples collected from an elderly care center. A total of 62 samples were measured, and in most cases, bcarotene (17.5-1105.7 ng/mL) was present in the highest amount, followed by lutein (10.6-182.3 ng/mL) and lycopene (0-402 ng/mL). 一種簡單快速的高效率液相層析法已被發展出，以測定人體血液中的主要類胡蘿蔔素。使用C18管柱配合等比動相甲醇／二氯甲烷（95:5, v／v）及樣品溶劑甲醇／二氯甲烷（45:55, v／v），偵測波長476nm和流速0.8 mL／min，可於20分鐘內分離出葉黃素、β-胡蘿蔔素及番茄紅素，且回收率分別為92, 90及87% 。使用此方法分析由老人安養中心收集的62個血液樣品，結果顯示大部分血液樣品具有最高量的β-胡蘿蔔素，次為葉黃素及番茄紅素。