應用基質固相分佈萃取技術於甘蔗中草滅淨殘留量之分析   全文下載

Determination of Simazine Residue in Sugarcane by Application of Matrix Solid Phase Dispersion (MSPD) Extraction Technique

本研究建立了應用基質固相分佈（matrix solid phase dispersion, MSPD）萃取技術及矽酸鎂固相萃取匣淨化，分析甘蔗檢體中草滅淨（simazine）殘留量之簡便的分析方法。此分析法於單一沖提步驟中，可同時進行萃取及淨化，大幅降低有機溶媒之使用量。取均質後之甘蔗檢體2 g，與2 gEnvirElut吸附劑研磨混合至均態分佈'將此基質混合物移至矽酸鎂固相萃取匣，以二氯甲烷25 mL分次沖提，沖提液經減壓濃縮至乾後以乙?溶解，最後以高效液相層析儀分析，偵測波長為230 nm。添加草滅淨0.1~0.5 ppm於甘蔗中，三重複之平均回收率為86.9~94.7%，變異係數小於10%。本方法之最低檢出限量為0.02 ppm。"

A rapid method for the determination of simazine residue in sugarcane by matrix solid phase dispersion (MSPD) extraction and florisil cleanup was developed. This method performed extraction and cleanup in a single eluting step and significantly reduced organic solvent consumption. Two grams of homogenized sample was blended with 2 g of EnvirElut sorbent until a homogeneous mixture was observed. The mixture was transferred into a florisil cartridge, and eluted with 25 mL of dichloromethane. The eluate was evaporated to dryness, and the residue was dissolved with acetonitrile and then determined by HPLC-UV at 230 nm. Recoveries of simazine spiked in sugarcane (0.1~0.5 ppm) were determined to be 86.9~94.7%, while coefficient of variation was determined to be less than 10%. The detection limit was 0.02 ppm. 本研究建立了應用基質固相分佈（matrix solid phase dispersion, MSPD）萃取技術及矽酸鎂固相萃取匣淨化，分析甘蔗檢體中草滅淨（simazine）殘留量之簡便的分析方法。此分析法於單一沖提步驟中，可同時進行萃取及淨化，大幅降低有機溶媒之使用量。取均質後之甘蔗檢體2 g，與2 gEnvirElut吸附劑研磨混合至均態分佈'將此基質混合物移至矽酸鎂固相萃取匣，以二氯甲烷25 mL分次沖提，沖提液經減壓濃縮至乾後以乙腈溶解，最後以高效液相層析儀分析，偵測波長為230 nm。添加草滅淨0.1～0.5 ppm於甘蔗中，三重複之平均回收率為86.9～94.7%，變異係數小於10%。本方法之最低檢出限量為0.02 ppm。