台灣地區食品中毒之金黃色葡萄球菌腸毒素C型菌株次分型分析   全文下載

Subtyping of Enterotoxin C Strains Isolated from Food Poisoning Outbreaks in Taiwan

金黃色葡萄球菌在世界各地均是重要的食品中毒原因菌，產生的腸毒素分為許多型別，腸毒素C型可細分為C1、C2、C3等次分型。除腸毒素外，金黃色葡萄球菌會產生多種胞外蛋白質及毒素，包含凝固?（coagulase）、溶血素（hemolysins）、溶菌素（lysostaphin）、核酸?（nuclease）、蛋白質A（protein A）等三十餘種，其中凝固?及蛋白質A之產生為金黃色葡萄球菌重要的生化特性之一。本研究收集台灣地區36個食品中毒案分離之229株金黃色葡萄球菌，利用聚合?鏈反應（PCR）方法進行腸毒素C型產毒菌基因之次分型，並以PCR-RFLP方法檢測腸毒素C型菌其凝固?及蛋白質A基因，進而加以分型。結果顯示由中毒案所分離的10株產腸毒素C型之金黃色葡萄球菌中有3株為腸毒素C2型，7株為C3型。凝固?基因以PCR方法偵測可區分為兩型，以限制?Aul I將PCR產物進行反應亦可分為兩型。蛋白質A基因分型不論以PCR或以限制?Hind III將PCR產物進行反應之結果均屬同一類型。本研究發展的快速分型檢測方法應用於食品中毒案可輔助污染源之分析。

Staphylococcus aureus is one of the important foodborne pathogens that caused food poisoning worldwide. There are several serologically distinct staphylococcal enterotoxins (A, B, C, D, E). SEC is subdivided into SEC1, SEC2, SEC3 based on their minor epitopes. Besides the enterotoxins, there are over thirty categories of extracellular proteins and toxins secreted by S. aureus, including coagulase, hemolysins, nuclease, and protein A. Among which, coagulase and protein A are critical biochemical characteristics of S. aureus. Total of 229 S. aureus strains were collected from 36 food poisoning outbreaks occurred in the Taiwan area and only ten SEC isolates were identified. Subtyping of these strains was analyzed by the polymorphism of the SEC, coagulase and protein A genes performed by polymerase chain reaction (PCR). Three strains belonged to SEC2 and seven belonged to SEC3 and PCR of the sec gene also showed to have subtypes. Two types of coagulase gene were detected by PCR and PCR-RFLP methods. Typing for the protein A gene by PCR revealed that the amplified, products digested by Hind III or not, were identical. These rapid subtyping methods developed in this study would be valuable in analyzing the sources of contaminated food in the poisoning outbreaks. 金黃色葡萄球菌在世界各地均是重要的食品中毒原因菌，產生的腸毒素分為許多型別，腸毒素C型可細分為C1、C2、C3等次分型。除腸毒素外，金黃色葡萄球菌會產生多種胞外蛋白質及毒素，包含凝固酶（coagulase）、溶血素（hemolysins）、溶菌素（lysostaphin）、核酸酶（nuclease）、蛋白質A（protein A）等三十餘種，其中凝固酶及蛋白質A之產生為金黃色葡萄球菌重要的生化特性之一。本研究收集台灣地區36個食品中毒案分離之229株金黃色葡萄球菌，利用聚合酶鏈反應（PCR）方法進行腸毒素C型產毒菌基因之次分型，並以PCR-RFLP方法檢測腸毒素C型菌其凝固酶及蛋白質A基因，進而加以分型。結果顯示由中毒案所分離的10株產腸毒素C型之金黃色葡萄球菌中有3株為腸毒素C2型，7株為C3型。凝固酶基因以PCR方法偵測可區分為兩型，以限制酶Aul I將PCR產物進行反應亦可分為兩型。蛋白質A基因分型不論以PCR或以限制酶Hind III將PCR產物進行反應之結果均屬同一類型。本研究發展的快速分型檢測方法應用於食品中毒案可輔助污染源之分析。