蔬菜中硝酸鹽及亞硝酸鹽含量之高效液相層析法定量分析   全文下載

A High Performance Liquid Chromatography Method for Determining Nitrate and Nitrite Levels in Vegetables

本研究係探討蔬菜中硝酸鹽及亞硝酸鹽之定量分析，發展出一種簡單、快速、精確及敏感度佳的高效液相層析方法（high performance liquid chromatography, HPLC ）。分析較適條件為以30% （v／v） 的甲醇水溶液添加0.01 M 辛胺 （octylammonium），並以正磷酸調整pH值至7.0，作為移動相溶液，流速為0.8 mL／min，每一樣品在十分鐘內即可分析完成。硝酸鹽及亞硝酸鹽之回收率介於96.6%至105.7%間，硝酸鹽及亞硝酸鹽於0.1~~100.0 mg／L 濃度範圍內之標準曲線線性迴歸係數均大於0.9990，線性關係非常佳。因此，高效液相層析法相當適用於定量分析蔬菜中硝酸鹽及亞硝酸鹽含量。將HPLC方法應用於檢測12種市售蔬菜，結果硝酸鹽及亞硝酸鹽含量範圍分別為225~4,410 mg／kg及＜5-200 mg／kg。"

In this study, a simple, rapid, precise and sensitive high performance liquid chromatography (HPLC) method using an UV detector was developed for the determination of nitrate and nitrite amounts in vegetables. The optimal conditions were found and applied using 0.01 M octylammonium orthophosphate of aqueous 30% (v/v) methanol of pH 7.0 for the mobile phase at flow rate of 0.8 mL/min. The total time for one sample analysis was within 10 min. Recoveries of nitrate and nitrite were between 96.6% to 105.7%. The calibration curves of nitrate and nitrite were extremely linear, where both correlation coefficients were greater than 0.9990 in the range of 0.1~100.0 µg/mL. Therefore, this HPLC method is applicable for simultaneously determining the nitrate and nitrite levels in vegetables. For application, nitrate and nitrite amounts in 12 marketed vegetables were determined by this HPLC method. The results showed nitrate and nitrite contents varied in a range of 225~4,410 mg/kg and <5~200 mg/kg, respectively. 本研究係探討蔬菜中硝酸鹽及亞硝酸鹽之定量分析，發展出一種簡單、快速、精確及敏感度佳的高效液相層析方法（high performance liquid chromatography, HPLC ）。分析較適條件為以30% （v／v） 的甲醇水溶液添加0.01 M 辛胺 （octylammonium），並以正磷酸調整pH值至7.0，作為移動相溶液，流速為0.8 mL／min，每一樣品在十分鐘內即可分析完成。硝酸鹽及亞硝酸鹽之回收率介於96.6%至105.7%間，硝酸鹽及亞硝酸鹽於0.1～100.0 mg／L 濃度範圍內之標準曲線線性迴歸係數均大於0.9990，線性關係非常佳。因此，高效液相層析法相當適用於定量分析蔬菜中硝酸鹽及亞硝酸鹽含量。將HPLC方法應用於檢測12種市售蔬菜，結果硝酸鹽及亞硝酸鹽含量範圍分別為225～4,410 mg／kg及＜5-200 mg／kg。