以石墨爐式原子吸收光譜儀直接分析食用油脂類中砷之含量   全文下載

Determination of Arsenic in Edible Oils by Direct Graphite Furnace Atomic Absorption Spectrometry

本研究探討以石墨爐式原子吸收光譜儀直接分析食用油脂類中之砷含量。將檢體以正庚烷稀釋後，以砷有機標準溶液（Arsenic organic AAS standard solution）為標準液，利用側向加熱石墨管及茲曼石墨爐式原子吸收光譜儀直接偵測檢體中砷之含量。實驗結果顯示，最適灰化溫度為1200°C，最適原子化溫度為2300°C，基質修飾劑則以二苯?二氯鈀效果最佳。本方法對油脂樣品中時之偵測極限為0.010 ppm。其次，添加砷0.05，0.10 及0.20 ppm於大豆沙拉油中，其回收率為91.2%~94.3%。添加於魚油中，回收率為94.7%-~95.6%。添加於棕櫚油中，其回收率為94.2%~96.7% 。添加於豬油中，回收率為93.3%-~94.1%。以此方法分析市售食用油脂30件，其分析結果均低於方法偵測極限。

A direct analytical method of arsenic content in edible oil with graphite furnace atomic absorption spectrophotometer was studied. Edible oil was diluted with n-heptane followed by the determination of arsenic with Zeeman graphite furnace atomic absorption spectrophotometer and transverse heated graphite atomizer (THGA). The arsenic organic AAS standard solution was used as calibration standard, and bis (benzonitrile) dichloro palladium solution was used as a matrix modifier. The results showed that the optimal ashing and atomizing temperatures were 1200ûC and 2300ûC, respectively. The detection limit was estimated to be 0.010 ppm, and the percentages of recovery for the spike of 0.05, 0.10 and 0.20 ppm arsenic to salad oil, fish oil, palm oil, and lard were 91.2~94.3%, 94.7~95.6%, 94.2~96.7% and 93.3~94.1%, respectively. With the use of this method, thirty edible oils were analyzed and the arsenic contents were found to be below the detection limits. 本研究探討以石墨爐式原子吸收光譜儀直接分析食用油脂類中之砷含量。將檢體以正庚烷稀釋後，以砷有機標準溶液（Arsenic organic AAS standard solution）為標準液，利用側向加熱石墨管及茲曼石墨爐式原子吸收光譜儀直接偵測檢體中砷之含量。實驗結果顯示，最適灰化溫度為1200°C，最適原子化溫度為2300°C，基質修飾劑則以二苯腈二氯鈀效果最佳。本方法對油脂樣品中時之偵測極限為0.010 ppm。其次，添加砷0.05，0.10 及0.20 ppm於大豆沙拉油中，其回收率為91.2%～94.3%。添加於魚油中，回收率為94.7%-～95.6%。添加於棕櫚油中，其回收率為94.2%～96.7% 。添加於豬油中，回收率為93.3%-～94.1%。以此方法分析市售食用油脂30件，其分析結果均低於方法偵測極限。