應用毛細管電泳線上樣品堆積法於柴胡皂苷分析   全文下載

Separation of Saikosaponins by On-line Sample Stacking CE Method

柴胡粗抽提液中微量的柴胡皂? a、c、d、b，及b2，可用毛細管電泳線上樣品堆積技術（掃集法）成功分離。該法利用pH低於2的磷酸水溶液，配製等導電度的萃取溶劑與緩衝溶液；前者用甲醇調節，後者加入SDS、甲醇和氰甲烷。以毛細管電泳掃集法分析柴胡藥材，其電泳圖比用MEKC分析柴胡濃縮液的教果更佳，本法有更高的分離效率、更低的偵測極限，並可測得五個柴胡皂?。用於以柴胡為君藥的小柴胡湯與大柴胡湯，亦能順利測得柴胡皂?a及b1；惟中藥製劑複雜的基質明顯影響指標成分的遷移時間與解析度。

Saikosaponins a, c, d, b1 and b2 which are the trace constituents in the crude extract of Bupleuri Radix can be separated successfully by the on-line sample stacking technique (Sweeping method) of capillary electrophoresis. The method uses aqueous phosphoric acid with a pH lower than 2 to prepare the extracting solvent and buffer solution of the same electric conductance. The former is prepared with methanol and the latter is prepared by adding SDS, methanol and acetonitrile. Bupleuri Radix extract analyzed by CE-sweeping method gives better result than by MEKC method with a higher separation efficiency, lower detection limit and ability to detect five saikosaponins. Applied to the analysis of two Chinese herbal formulas, CE-sweeping method is found to be able to determine the contents of saikosaponins a and b1 successfully in Minor Bupleurum Combination and Major Bupleurum Combination, both of which contain Bupleuri Radix as the imperial drug. However, the migration times and resolution of the two marker substances, saikosaponins a and b1, were changed obviously by the complicate constituents in Chinese herbal preparations. 柴胡粗抽提液中微量的柴胡皂苷 a、c、d、b，及b2，可用毛細管電泳線上樣品堆積技術（掃集法）成功分離。該法利用pH低於2的磷酸水溶液，配製等導電度的萃取溶劑與緩衝溶液；前者用甲醇調節，後者加入SDS、甲醇和氰甲烷。以毛細管電泳掃集法分析柴胡藥材，其電泳圖比用MEKC分析柴胡濃縮液的教果更佳，本法有更高的分離效率、更低的偵測極限，並可測得五個柴胡皂苷。用於以柴胡為君藥的小柴胡湯與大柴胡湯，亦能順利測得柴胡皂苷a及b1；惟中藥製劑複雜的基質明顯影響指標成分的遷移時間與解析度。