中草藥中摻加Prednisone 之固相萃取與高效液相層析定量分析   全文下載

Solid-phase Extraction and High-performance Liquid Chromatographic Analysis of Prednisone Adulterated in a Foreign Herbal Medicine

一種來自東南亞的中草藥檢體，以薄層層析及紫外分光光度計檢驗出含prednisone成分，經固相萃取管處理後，再以高效液相層析法定量。固相萃取管依序以甲醇及氯仿先活化，繼用二氯甲烷及異丙醇（6:4, v／v）沖提檢體。高效液相層析採用逆相層析管柱（Inertsil ODS-80A），沖提液為乙?與水（3:7, v／v），檢測波長為240 nm，內部標準品為fludrocortisone acetate。Prednisone檢量線之濃度範圍為10.0-200.0μg／mL。確效結果顯示精密度、準確度及回收率均良好。以上述方法定量分析檢出每包檢體（約21公克）含prednisone 5.5毫克。"

Prednisone was detected in a herbal medicine from Southeast Asia using thin layer chromatography (TLC) and ultraviolet spectroscopy (UV). After treating with solid-phase extraction (SPE), the sample was further assayed by high-performance liquid chromatography (HPLC). The sample solution was achieved with a silica gel cartridge conditioned with methanol and chloroform in sequence, and then eluted with dichloromethane-isopropanol (6:4, v/v). Separation was conducted with an Inertsil ODS-80A reversed-phase column using isocratic elution with acetonitrile and water (3:7, v/v) as mobile phase. Fludrocortisone acetate was used as an internal standard and detection wavelength was at 240 nm. Calibration curve of standard prednisone was constructed in the range of 10.0-200.0 μg/mL. The content of adulterated prednisone was 5.5 mg/21g sample. 一種來自東南亞的中草藥檢體，以薄層層析及紫外分光光度計檢驗出含prednisone成分，經固相萃取管處理後，再以高效液相層析法定量。固相萃取管依序以甲醇及氯仿先活化，繼用二氯甲烷及異丙醇（6:4, v／v）沖提檢體。高效液相層析採用逆相層析管柱（Inertsil ODS-80A），沖提液為乙腈與水（3:7, v／v），檢測波長為240 nm，內部標準品為fludrocortisone acetate。Prednisone檢量線之濃度範圍為10.0-200.0μg／mL。確效結果顯示精密度、準確度及回收率均良好。以上述方法定量分析檢出每包檢體（約21公克）含prednisone 5.5毫克。