| 英文摘要 |
Baicalin, baicalein and wogonin are bioactive flavonoids of Scutellariae Radix. In order to compare the contents of baicalin, baicalein and wogonin between traditional decoction and commercial extract, ten crude drugs and ten commercial extracts of Scutellariae Radix were purchased from the market. High performance liquid chromatographic method was used for simultaneous assay of these flavonoid constituents. The separation was performed on an Inertsil ODS-2 column with acetonitrile - 0.005% phosphoric acid (36:64, v/v) as mobile phase at a flow-rate of 1.0 mL/min and detection at 270 nm, and ethyl paraben was used as the internal standard. This method is applicable for the routine quality control of the crude drug and commercial extract of Scutellariae Radix. The results indicated that the contents of baicalin, baicalein and wogonin in traditional decoctions of each gram of Scutellariae Radix were 62.7 ± 4.6 mg, 5.5 ± 0.2 mg and 1.7 ± 0.1 mg, whereas those in each gram of commercial extracts were 82.3 ± 8.5 mg, 15.0 ± 2.0 mg and 5.3 ± 0.5 mg, respectively. The suggested daily doses of crude drug and commercial extract of Scutellariae Radix are 3.0 – 9.0 g and 0.4 – 9.0 g, respectively. Based on our results, the contents of baicalin, baicalein and wogonin in the maximum daily doses for traditional decoctions of Scutellariae Radix were 564.7 ± 41.7 mg, 49.7 ± 1.9 mg and 15.6 ± 1.0 mg, whereas those for commercial extracts were 241.9 ± 41.1 mg, 53.7 ± 16.4 mg and 17.9 ± 4.6 mg, respectively.
本研究使用等壓高效液相層析法，同時分析黃芩傳統煎劑及濃縮製劑中之黃酮類成分黃芩苷、黃芩苷元和漢黃芩素之含量。採用Inertsil ODS-2逆相層析管柱，以acetonitrile - 0.005% phosphoric acid （36:64, v／v）之混合溶液為移動相，流速為1 mL／min，檢測波長為270nm，並以ethyl paraben為內標準。此分析方法可應用於黃芩藥材和濃縮製劑產品之品質管制。定量分析各十個市場品之結果顯示，每公克黃芩之傳統煎劑含黃芩苷、黃芩苷元及漢黃芩素平均分別為62.7 ± 4.6、5.5 ± 0.2及1.7± 0.1毫克，而濃縮製劑中每公克平均含82.3 ± 8.5、15.0 ± 2.0及5.3 ± 0.5毫克。計算其每日最大劑量，黃芩傳統煎劑平均含黃芩苷、黃芩苷元及漢黃芩素分別為564.7 ± 41.7、49.7 ± 1.9及15.6 ± 1.0毫克，而濃縮製劑則含241. 9 ± 41.1、53.7 ± 16.4及17.9 ± 4.6毫克。 |
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