以RAPD法鑑定山藥藥材之基原   全文下載

Genetic Identification of Chinese Drug Materials in Yams (Dioscorea spp.) by RAPD Analysis

以RAPD法檢測薯蕷屬（Dioscorea）18個品系山藥藥材的基原。以30個隨機引子進行聚合?連鎖反應增幅山藥藥材葉子之DNA。30個引子中的18個引子可產生237個基因標誌。其中的四個引子OPP-40, OPP-15，OPAP-04及OPAP-10可用於區分這18個品系之山藥藥材。計算品系間之相似與相異係數及分群分析可建構品系間之基因親緣關係圖，並可將18個品系分為5個次群。基因相似及相異度分析顯示70W37品系與70W47品系差異較大，而70W41品系則與70W59品系較為相似。本研究已成功的應用RAPD法鑑定及區別薯蕷屬的18個品系山藥藥材。"

The botanical origins of Chinese drug materials in yams were examined among eighteen cultivarities/ species of Dioscorea by randomly amplified polymorphic DNA (RAPD). DNA isolated from fresh leaves were used as a template in polymerase chain reactions (PCR) using thirty random decamer primers. Eighteen primers produced 237 polymorphic RAPD markers, which were scored for all the lines studied. Four primers including OPP-04, OPP-15, OPAP-04 and OPAP-10, could distinguish all tested samples. Genetic distances and similarities among eighteen cultivarities/species were calculated, and a cluster analysis was used to generate a dendrogram showing a phylogenetic relationship. The studied Dioscorea species could be divided into five subgroups by phylogenetic analysis. Genetic distance and similarity analysis showed that line 70W37 was distantly related to 70W47, and line 70W41 was closely related to 70W59. RAPD technique could be successfully applied for the rapid identification and differentiation of Dioscorea species. 以RAPD法檢測薯蕷屬（Dioscorea）18個品系山藥藥材的基原。以30個隨機引子進行聚合酶連鎖反應增幅山藥藥材葉子之DNA。30個引子中的18個引子可產生237個基因標誌。其中的四個引子OPP-40, OPP-15，OPAP-04及OPAP-10可用於區分這18個品系之山藥藥材。計算品系間之相似與相異係數及分群分析可建構品系間之基因親緣關係圖，並可將18個品系分為5個次群。基因相似及相異度分析顯示70W37品系與70W47品系差異較大，而70W41品系則與70W59品系較為相似。本研究已成功的應用RAPD法鑑定及區別薯蕷屬的18個品系山藥藥材。