黃柏素-7-葡萄糖之代謝物在大白鼠腦中之分析及動力學   全文下載

Determination and Disposition Kinetics of Phellamurin Metabolites in Rat Brain

黃柏素-7- 葡萄糖?是台灣黃蘗的樹葉中含量頗豐的一種雙氫黃酮類配醣體。本研究探討黃柏素-7- 葡萄糖?之代謝物在大鼠腦中之動力學。大鼠口服 100 mg／kg 和 200 mg／kg 兩種劑量，於給藥後0.33，2，4及6小時將大鼠犧牲，分析腦中黃柏素-7- 葡萄糖?之代謝物。定量方法係採用高效液相層析法，檢測腦組織經β-葡萄糖醛酸?水解之前及之後所含新黃柏素之濃度。移動相為乙?：水（含1% 醋酸，38 : 62，v／v）。結果顯示新黃柏素葡萄糖醛酸可能穿越血腦障蔽，其腦中之濃度呈現劑量相關性，而新黃柏素在腦中的含量則極微。

Phellamurin is a flavanone glycoside that is abundant in the leaves of Phellodendron wilsonii Hayata et Kanehira (Rutaceae). The disposition kinetics of phellamurin metabolites in rat brain was investigated in this study. Doses of 100 mg/kg and 200 mg/kg were orally administered to rats. At 0.33, 2, 4 and 6 hr after dosing, rats were dissected and the brain concentrations of the aglycone neophellamuretin were determined by an HPLC method prior to and after hydrolysis with β-glucuronidase. The mobile phase was acetonitrile: H2O (containing 1% acetic acid, 38:62, v/v). Our results indicated that neophellamuretin glucuronide might be able to cross the blood-brain barrier and its concentration in the brain was dose-dependent at the two doses investigated, whereas the aglycone neophellamuretin was present in a trace amount in the brain. 黃柏素-7- 葡萄糖苷是台灣黃蘗的樹葉中含量頗豐的一種雙氫黃酮類配醣體。本研究探討黃柏素-7- 葡萄糖苷之代謝物在大鼠腦中之動力學。大鼠口服 100 mg／kg 和 200 mg／kg 兩種劑量，於給藥後0.33，2，4及6小時將大鼠犧牲，分析腦中黃柏素-7- 葡萄糖苷之代謝物。定量方法係採用高效液相層析法，檢測腦組織經β-葡萄糖醛酸酶水解之前及之後所含新黃柏素之濃度。移動相為乙腈：水（含1% 醋酸，38 : 62，v／v）。結果顯示新黃柏素葡萄糖醛酸可能穿越血腦障蔽，其腦中之濃度呈現劑量相關性，而新黃柏素在腦中的含量則極微。