市售維生素飲料及口服液中菸鹼醯胺之直接注入氣相層析快速定量分析法   全文下載

A Rapid and Simple Gas Chromatographic Method for Direct Determination of Nicotinamide in Commercial Vitamins and Tonic Drinks

本研究建立了直接注入氣相層析分析市售胺基酸飲料、維生素飲料、雞精及口服液等液體樣品中菸鹼醯胺之快速、簡便之測定方法。採用直接注入（direct injection）之方式，以中間極性之CP-SIL 8CB 管柱（30 m × 0.53 mm）分析定量上述液體樣品之菸鹼醯胺，選擇水溶性之1,9-壬二醇（1,9-nonanediol）為內標準，最低檢出量約為2-5 μg／mL 左右。添加菸鹼醯胺105.5 及211.0 μg 於1 mL 維生素飲料及口服液檢體中，直接注入GC 分析，其回收率分別為94-99%及93-108%，變異係數均在9.8%以下。以本方法分析市售不同廠牌之胺基酸飲料、維生素飲料、雞精及口服液等液體樣品共42 件之菸鹼醯胺含量分別為131-246 、24-112 、0-134 及0-263 μg／mL 。相當於每瓶上述檢體中分別含菸鹼醯胺39-74 、5-22 、0-10 及0-21 mg／bottle 。此結果顯示， 12 件胺基酸及維生素飲料中，每瓶菸鹼醯胺含量有4 件超過國人每日平均建議攝取量（RNDA ＝ 14.4 mg）許多；而10 件口服液中每瓶菸鹼醯胺亦有3 件超過國人每日平均建議攝取量。"

A simple and rapid method was developed to determine the presence of nicotinamide in tonic drinks using the megapore semi-polar column (CP-Sil 8 CB, 30 m × 0.53 mm) with direct injection gas chromatography. Direct quantitative analysis of nicotinamide in amino acid drinks, vitamin drinks, essence of chicken and tonic drinks was carried out without any sample pretreatment procedure. Water soluble compound 1,9-nonanediol was used as an internal standard. The detection limit for nicotinamide was 2-5 μg/mL. Recovery studies were performed using 1 mL of vitamin drink and tonic drink, each spiked with nicotinamide at 105.5, and 211.0 μg, respectively. The recoveries were found in the range of 94~99% and 93- 108%, respectively. The coefficients of variation being less than 9.8%. Forty-two commercial amino acid drinks, vitamin drinks, essences of chicken and tonic drinks were analyzed with this method. The nicotinamide contents were found as: 131-246, 24-112, 0-134 and 0-263 μg/mL, respectively. These results indicated that, one bottle each of the amino acid drink, vitamin drink, essences of chicken and tonic drink contain nicotinamide levels of : 39-74, 5-22, 0- 10 and 0-21 mg/bottle. The results also showed that in 12 out of 4 of commercial amino acid and vitamin drinks, the nicotinamide exceeded the ROC’s acceptable daily intake (RNDA, 14.4 mg) level; and 3 out of 10 commercial tonic drinks exceeded the ROC’s RNDA level for nicotinamide. 本研究建立了直接注入氣相層析分析市售胺基酸飲料、維生素飲料、雞精及口服液等液體樣品中菸鹼醯胺之快速、簡便之測定方法。採用直接注入（direct injection）之方式，以中間極性之CP-SIL 8CB 管柱（30 m × 0.53 mm）分析定量上述液體樣品之菸鹼醯胺，選擇水溶性之1,9-壬二醇（1,9-nonanediol）為內標準，最低檢出量約為2-5 μg／mL 左右。添加菸鹼醯胺105.5 及211.0 μg 於1 mL 維生素飲料及口服液檢體中，直接注入GC 分析，其回收率分別為94-99%及93-108%，變異係數均在9.8%以下。以本方法分析市售不同廠牌之胺基酸飲料、維生素飲料、雞精及口服液等液體樣品共42 件之菸鹼醯胺含量分別為131-246 、24-112 、0-134 及0-263 μg／mL 。相當於每瓶上述檢體中分別含菸鹼醯胺39-74 、5-22 、0-10 及0-21 mg／bottle 。此結果顯示， 12 件胺基酸及維生素飲料中，每瓶菸鹼醯胺含量有4 件超過國人每日平均建議攝取量（RNDA ＝ 14.4 mg）許多；而10 件口服液中每瓶菸鹼醯胺亦有3 件超過國人每日平均建議攝取量。