人體尿液及血液中殺草劑本達隆及其代謝產物之分析   全文下載

Analysis of Bentazon and Its Metabolites in Urine and Blood

本研究進行血液及尿液中殺草劑類農藥本達隆（Bentazon），及其代謝物6-hydroxybentazon及8-hydroxy-bentazon 快速分析方法之研究。利用固相萃取法（solid phase extraction）及高效液相層析儀附紫外光偵測器（HPLC／UV）來進行全自動之萃取分析。尿液中各加入2μg 之方法回收率分別為62.16±3.05%（Bentazon）， 59.01 ± 3.19%（6-OH-bentazon），109.93 ± 0.01%（8-OH-bentazon）。而血液中之回收率分別為119.45 ± 3.46%（Bentazon）， 138.37 ± 6.72%（6-OH-bentazon）， 131.02 ± 3.86%（8-OH-bentazon）。1 mL尿液樣品之偵測界限分別為0.047ppm（bentazon）， 0.052ppm（6-OH-bentazon）， 0.024ppm（8-OH-bentazon）。另外，可利用氣相層析質譜儀（GC／MS），以選擇性離子檢測方式（SIM）來進行本達隆之鑑定分析。我們利用此快速之萃取分析方法來進行89 件人體農藥中毒送檢樣品之分析，包括尿液51 件，血清30 件，全血4 件，洗腎水4 件，分別測得之濃度範圍為0 ~ 1682.43ppm（bentazon）， 0 ~ 617.64ppm（6-OH-bentazon）， 0 ~ 4.12ppm（8-OH-bentazon）。 A rapid analysis method of herbicide bentazon and its metabolites (6-OH-bentazon and 8- OH-bentazon) in urine and blood has been developed. Automatic solid phase extraction and HPLC/UV were used to extract and analyze pesticides. The recoveries of bentazon, 6-OH-bentazon and 8-OH-bentazon in urine spiked with 2 μg of standards were 62.16 ± 3.05%, 59.01 ± 3.19%, and 109.93 ± 0.01%, respectively. In blood, the recoveries were 119.45 ± 3.46%, 138.38 ± 6.72%, and 131.02 ± 3.86%, respectively. The detection limits of bentazon, 6-OH-bentazon and 8-OH-bentazon in urine are 0.047, 0.052, and 0.024 ppm, respectively. Gas chromatography- mass spectrometry (GC-MS) was also used for further identification of bentazon. We have used this method to extract and analyze bentazon in biological samples. In 89 samples examined, including 51 samples of urine, 30 samples of serum, 4 samples of blood, and 4 samples of renal water, we detected bentazon, 6-OH-bentazon and 8-OH-bentazon. The range of concentration was 0~1682.43 ppm."

A rapid analysis method of herbicide bentazon and its metabolites (6-OH-bentazon and 8- OH-bentazon) in urine and blood has been developed. Automatic solid phase extraction and HPLC/UV were used to extract and analyze pesticides. The recoveries of bentazon, 6-OH-bentazon and 8-OH-bentazon in urine spiked with 2 μg of standards were 62.16 ± 3.05%, 59.01 ± 3.19%, and 109.93 ± 0.01%, respectively. In blood, the recoveries were 119.45 ± 3.46%, 138.38 ± 6.72%, and 131.02 ± 3.86%, respectively. The detection limits of bentazon, 6-OH-bentazon and 8-OH-bentazon in urine are 0.047, 0.052, and 0.024 ppm, respectively. Gas chromatography- mass spectrometry (GC-MS) was also used for further identification of bentazon. We have used this method to extract and analyze bentazon in biological samples. In 89 samples examined, including 51 samples of urine, 30 samples of serum, 4 samples of blood, and 4 samples of renal water, we detected bentazon, 6-OH-bentazon and 8-OH-bentazon. The range of concentration was 0~1682.43 ppm.