不同油脂魚體中有機氯及含氮農藥殘留偵測   全文下載

Determination of Organochlorine and Nitrogen-Containing Pesticide Residues in Fish with Different Fat Content

建立一個快速的、利用多種殘留（multiresidue）分析及固相萃取（solid-phsae extraction）技術，偵測不同油脂含量魚體中有機氯及含氮農藥的方法。在此方法中，樣品以乙抽出，萃取物利用以C18 和矽酸鎂（florisil）管柱串聯的固相萃取管淨化，乙為淨化過程中唯一使用的溶劑。殘留藥劑利用氣相層析儀（gas chromatography, GC）附電子捕擭式檢出器（electron capture detector, ECD）偵測。回收率試驗以12 種有機氯農藥及其代謝產物（aldrin, chlorobenzilate, dieldrin, endosulfan sulfate, endosulfan, endrin, heptachlor, heptachlor epoxide, lindane, methoxchlor, p,p’-DDE, p,p’-DDT）和2 種含氮農藥（alachlor, trifluralin）共14 種藥劑的不同濃度組合（一倍、兩倍及四倍添加量），在9 種油脂含量範圍為1.2??3.3%的魚體中分別進行。魚體依油脂含量分為三組（油脂含量＜ 10%、≧ 10%但＜ 20%及≧ 20%）的試驗結果中，除了可能有一組樣品魚是已受到p,p’籺DT 污染、有一組樣品魚可能受到endosulfan 污染或圖譜受到基質干擾外，其餘樣品魚體內供試藥劑回收率在73.4%至119.6%間，且回收率在不同處理濃度及不同油脂含量魚體間並無顯著差異。"

A rapid and multiresidue method for determination the residues of organochlorine and nitrogen-containing pesticides in the different fat content fish by solid-phase extraction (SPE) technique was developed. In the present procedures, samples were extracted with acetonitrile. The extracts were cleaned up by a tandem SPE column of C18 and florisil, using acetonitrile as the only solvent. Residues were determined by gas chromatography equipped with electron capture detector. Recovery was obtained by three concentration levels of 12 organochlorine pesticides and their metabolites (chlorobenzilate, dieldrin, endosulfan sulfate, endosulfan, endrin, heptachlor, heptachlor epoxide, lindane, methoxchlor, p,p’-DDE, p,p’-DDT) and two nitrogen-containing pesticides (alachlor and trifluralin) from 9 fishes with 1.2 - 23.3% of fat content, respectively. The recoveries of the spiked pesticides residues from the fishes of different fat contain (fat < 10%, fat ≥ 10 but < 20%, and fat ≥ 20%) were in the range from 73.4% to 119.6%. There were no significant difference in the recovery between pesticides concentration and fat content. The recoveries of two samples were not included because of contaminated by p,p’-DDT and endosulfan separately, or disruption by sample matrix. 建立一個快速的、利用多種殘留（multiresidue）分析及固相萃取（solid-phsae extraction）技術，偵測不同油脂含量魚體中有機氯及含氮農藥的方法。在此方法中，樣品以乙抽出，萃取物利用以C18 和矽酸鎂（florisil）管柱串聯的固相萃取管淨化，乙為淨化過程中唯一使用的溶劑。殘留藥劑利用氣相層析儀（gas chromatography, GC）附電子捕擭式檢出器（electron capture detector, ECD）偵測。回收率試驗以12 種有機氯農藥及其代謝產物（aldrin, chlorobenzilate, dieldrin, endosulfan sulfate, endosulfan, endrin, heptachlor, heptachlor epoxide, lindane, methoxchlor, p,p’-DDE, p,p’-DDT）和2 種含氮農藥（alachlor, trifluralin）共14 種藥劑的不同濃度組合（一倍、兩倍及四倍添加量），在9 種油脂含量範圍為1.2􀀀3.3%的魚體中分別進行。魚體依油脂含量分為三組（油脂含量＜ 10%、≧ 10%但＜ 20%及≧ 20%）的試驗結果中，除了可能有一組樣品魚是已受到p,p’籺DT 污染、有一組樣品魚可能受到endosulfan 污染或圖譜受到基質干擾外，其餘樣品魚體內供試藥劑回收率在73.4%至119.6%間，且回收率在不同處理濃度及不同油脂含量魚體間並無顯著差異。