以電導法檢測旗魚肉中之生菌數及大腸桿菌群   全文下載

Determination of Aerobic Plate Count and Coliforms in Raw Swordfish Fillet by Conductimetric Method

本研究探討電導法（conductimetric method）應用於旗魚生魚片中生菌數和大腸桿菌群（coliforms）快速檢測之可行性。應用Malthus 2000 （Malthus Instruments Limited、Crawley，England）測得之電導檢測時間（detection time）和生菌數及大腸桿菌群數目之對數值相關性良好，其線性迴歸係數分別為－0.87 及－0.92。電導法具有快速及自動化之優點，可以做為旗魚生魚片中生菌數及大腸桿菌群快速檢測之用。

The feasibility of using a conductimetric method for determination of aerobic plate count and coliforms of swordfish fillet was investigated. Based on the change of conductance in the incubation cells, the relationship between the detection times and total plate count or coliforms was established by the Malthus 2000 (Malthus Instruments Limited, Crawley, England) system. The linear correlation coefficient between the log value of aerobic plate count of swordfish fillet and the detection time was -0.87, while the linear correlation coefficient between the log value of most probable number (MPN) of coliforms in the fish and the detection time was -0.92. Conductance measurement has the advantages of rapidity, automation and high capacity for analysis. Therefore, the technique can be used as an alternative method to conventional plate counting and the most probable number method for determination of aerobic plate count and coliforms, respectively, in swordfish fillet.