本土分離之Non-01霍亂弧菌 類霍亂毒素之生產與特性   全文下載

Production and Characterization of Cholera toxin-like Enterotoxin by a Non-01 Vibrio cholerae Isolated from Food

Non-01之霍亂弧菌能產生類霍亂毒素、耐熱腸毒素、類耐熱溶血素等毒素，引起腸炎。我們從台灣地區水產食物中分離到一株能產生類霍亂毒素的Non-01菌株，本篇報導該毒素的生產、細胞毒性與耐熱性。最適合毒素生產的培養條件為：casamino acid yeast extract lincomycin培養基、30°C、低震盪速率或靜止培養。該毒素不耐熱，在100°C加熱十分鐘後便喪失活性。在Chinese Hamster Ovary細胞測驗中顯示，該毒素的腸毒活性與霍亂毒素類似，其活性同時可被抗霍亂毒素抗血清所中和；在double diffusion試驗中該毒素卻不能與抗霍亂毒素抗血清形成作用線，因此該毒素應與霍亂毒素類似但不完全相同。

Non-01 V. cholerae caused small localized outbreaks of diarrhoeal diseases and produced cholera toxin-like enterotoxins, heat-stable enterotoxins and thermostable direct hemolysin-like hemolysins. In this paper, we reported on the production of a cholera toxin-like enterotoxin of a non-01 strain isolated form food in Taiwan and the bioactivity and themostability of this toxin. Production of toxin was optimum by stationary culture in casamino acid yeast extract lincomycin medium incubated at 30°C. This toxin was heat-labile and its cytotonic activity as assayed by Chinese hamster ovary cell was neutralized by anti-cholera toxin antiserum. However, this toxin failed to produce lines of identity with the anti-cholera toxin antiserum in a double gel diffusion assay.