大黃素在血清、藥材及中藥方劑中之高效層析定量   全文下載

HPLC Analysis of Emodin in Serum, Herbs and Chinese Herbal Prescriptions

大黃素為中藥大黃、虎杖、何首烏及決明子的活性成分之一。本研究利用高效液相層析法建立了大黃素在血清、中藥材（大黃、虎杖、何首烏、決明子）及四種中藥方劑（調胃承氣湯，小承氣湯，桃仁承氣湯及大承氣湯）中之快速簡便定量法，內標準選用2- 甲基蔥?。分離管柱採用RP-18。移動相溶媒為氯甲烷及2%醋酸水溶液，其最佳比例用於血清試樣為73：27，用於中藥試樣為55：45。移動相流速分別為0.9毫升／分及1.5毫升／分，檢測波長為280 nm，大黃素／2-甲基蔥?於血清試樣中的滯留時間為6.4分／9.1分，於中藥試樣中的滯留時間為8.3分／13.0分。此高效液相層析定量法精確性頗佳。本研究結果可應用於大黃素的藥物動態學研究、含大黃素中藥材之規格化及含大黃、虎杖、何首烏及決明子等中藥材的中藥方劑之品質管制。

Rapid and simple methods for the analysis of emodin in serum, herbs-Da Huang(大黃), Huu Jahng(虎杖), Her Shoou U(何首烏), Jyuer Ming Tzyy(決明子)and four Chinese herbal prescriptions- Tiaowei Chengqi Tang(調胃承氣湯), Xiao Chengqi Tang(小承氣湯), Taoren Chengqi Tang (桃仁承氣湯), Da Chengqi Tang(大承氣湯) were developed by application of reversed phase HPLC system. The HPLC conditions include a RP-18 column; elution with the mixture of MeCN and 2% HOAc; detection at 280 nm and using 2-methylanthraquinone as the internal standard. We observed that the optimum elution ratio(MeCN: 2 % HOAc) and flow rates for serum samples are 73:27 and 0.9 ml/min, respectively, while those for herbs and Chinese herbal preparations are 55:45 and 1.5 ml/min, respectively. The retention times of emodin/internal standard are 6.4 min/9.1 min and 8.3 min/13.0 min, respectively, for these two elution conditions. The established HPLC methods are accurate and precise. They could be uselful in pharmacokinetic study of emodin, quality evaluation of herbs containing emodin and quality control of Chinese herbal preparations including Da Huang as their component.