以溶劑萃取及氣相層析法快速測定屍體大腦中之乙醇含量   全文下載

The Rapid Determination of Ethanol in Postmortem Brain Samples by Solvent Extraction and Gas Chromatography

測定大腦中乙醇濃度常使用大腦組織蒸餾法，然而在法醫實驗室所使用的蒸餾法既費時又累贅，本研究係發展一套新穎又快速的方法以測定大腦組織中乙醇之含量。本方法是以n-butanol為萃取溶劑，繼之以n-propanol為內部對照標準品作氣相層析，結果發現此法之靈敏度與蒸餾法之靈敏度相近，並具有快速測定與容易定量之優點。

Distillation of brain tissue is a commonly used technique in the determination of brain ethanol concentrations. However, distillation suffers the disadvantages of being both time consuming and cumbersome in the forensic laboratory. In this study, we present a new, rapid method for the determination of ethanol content in brain tissue samples. This method employs solvent extraction with nbutanol and subsequent gas chromatographic analysis using n-propanol as an internal standard. The sensitivity of this technique was found to be similar to that obtained by distillation. The solvent extraction method presented has the advantages of rapidity of assay and ease of quantitation.