中藥製劑安中散之定量研究   全文下載

Quantitative Analysis of Cardamon and Fennel Combination

安中散為治氣血刺痛之重要方劑，含桂枝、縮砂、玄胡索、良薑、牡蠣、甘草、小茴香等藥材，本文以君藥桂枝的肉桂醛、香豆素、肉桂酸，及使藥甘草的甘草酸為指標，利用高效能液相層析儀評估中藥製劑的品質，並探討不同配伍、添加量、炮炙品對整體方劑的影響。實驗數據顯示，以水煎煮時，精油成分的抽出量很少，經濃縮續有大量損失；含桂枝之安中散的香豆素、肉桂醛含量高於桂枝單獨煎煮者，添加桂皮時結果相反，兩者裂成的製劑有很大不向；添加牡蠣可改變溶液的pH值，對肉桂酸、甘草酸的萃取率有很大增進作用；缺甘草或桂枝則各指標成分含量均減少，減去小茴香則各成分抽出量明顯增加；添加桂皮的炮炙品，對方劑的綜合化學成分含量有影響。藥材選用、方劑配伍、製作過程若不同，則製劑成分含量會有明顯差異，因此，市售製劑與自配濃縮浸膏有很大不同。

A Cardanon and Fennel combination is a traditional Chinese herbal formula for treating chi and blood - associated pricking pain. The formula is composed of Cinnamomi Ramus, Amomi Semen, Corydalis Tuber, Alpiniae Officinari Rhiwma, Ostreae Testa, Glycyrrhizae Radix, and Foeniculi Fructus. With the aid of high- performance liquid chromatography, we used cinnamaldehyde, coumarin, cinnamic acid (from the imperial component herb Cinnamomi Ramus), and glycyrrhizin (form the servant component herb Glycyrrhizae Radix) as the indicative constituents for the assessment of the quality of Chinese herb preparations and also for investigating the influences of different herb combinations, different doses, and different processed samples to the whole formula. Experimental data showed that when boiled in water, the formula yielded very little essential oil extract which, after concentration, this resulted in loss of more oil. Extractives of coumarin and cinnamaldehyde from Cardamon and Fennel combination which contains Cinnamomi Ramus were higher than from Cinnamomi Ramus decocted alone. The addition of Cinnamomi Cortex produced a contrary effect. Both preparations gave rise to greatly different results. The addition of Ostreae Testa changed the solution's pH value and promoted the exeraction yields of cinnamic acid and glycyrrhizin. Absence of Glycyrrhizae Radix or Cinnamomi Ramus caused reduction in the individual indicative constituents. Whereas, subtracting Foeniculi Fructus from the formula resulted in a marked increase in the extractives of the various constituents, and the addition of processed Cinnamomi Ramus produced a comprehensive influence of the chemical constituents. Differences in the drug materials selected, component combinations, and manufacturing processes produced marked differences in the constituent yields. Hence, there is a great difference found between commercial herbal products and laboratory - made counterparts.