以高效能液相層析法分析草蝦頭中紅色素   全文下載

Analysis of Carotenoids in Grass Prawn Heads by High Performance Liquid Chromatography

本文探討草蝦頭中類胡蘿蔔素之組成，以作為日後應用草蝦頭為天然紅色素之參考。以丙酮及石油醚促取草蝦頭，所得的紅色油狀萃取物，經低溫丙酮沉澱處理後，利用高效能液相層析法分析草蝦頭之紅色素組成及其相對含量。所用之分離管柱為Sumipax OA-2000（5μm, 0.46 cm i.d. × 25 cm），移動相為正己烷、二氯甲烷及乙醇，流速是1.5ml／min，在檢出器波長為470nm下，分析草蝦頭中紅色素。結果發現：1. 草蝦頭中紅色素之組成為astaxanthin （36.1 %），astaxanthin monoester （31.5%）及astaxanthin diester （17.5 %），尚有14.9%的色素未鑑定出。2. Astaxanthin部份，含有三種光學異構物，分別是：（3R, 3'R）-astaxanthin （15%）, （3R, 3'S）-astaxanthin （43.7%）及（3, 3'S）-astaxanthin（41.3 %）。"

The carotenoid composition in grass prawn (Penaeus monodon)heads was studied in this investigation to obtain basic information for any future application of the natural colorants from grass prawn heads. The red oily pigment in grass prawn heads was extracted with acetone, followed by petroleum ether (b. p. 30～50°C)which was dissolved in acetone and stored overnight at - 10°C. Next, the crude pigment was obtained by removal of precipitates from the cold acetone, then finally, removal of the acetone. The crude pigment was analyzed by HPLC with Sumipax OA-2000 column (5µm, 0. 46m i. d. × 25cm) and n-hexane/ dichloromethane/ ethanol (120: 17: 1, v/v/v)as a mobile phase. Flow rate was set at 1.5 ml/min and the dectector was set at 470 nm. The main results are shown as follows: 1. The main carotenoids in the grass prawn heads are composed of astaxanthin, astaxanthin monoester, astaxanthin diester, and some unidentified pigments. The relative amounts; of these pigments are 36.1%, 31.5%, 17.5%, and 14.9% respectively. 2. The astaxanthin fraction was separated into its three optical isomers: (3R, 3'R)-astaxanthin, (3R, 3'S)-astaxanthin, and (3S, 3'S)-astaxanthin. The relative amounts are 15%, 43.7%, and 41.3%, respectively, as determined by HPLC.