Background: Thirty two patients have been successfully treated by Peripheral Blood Stem Cell Transplantation (PBSCT) since 2008 till now. An investigation of 15 patient's self-care knowledge of PBSCT were extracted from the 32 patients that the correct rate of knowledge was only 67.9%. The possible causes of low self-care knowledge were lack of health education bulletins, complicated knowledge about PBSCT care lack of time and standardized process for health education, and insufficient follow-up strategies. In order to rebuild the patient's immunization functions in the duration of approximately one-year-period of time. It would be very important for patients to have sufficient knowledge of self-care. Purpose: The purpose of this project was to improve the self-care knowledge of patients with PBSCT and to decrease risks of infections. The cognition control threshold was 85%, the goal of this project was to achieve 85% regarding self care knowledge while patients with PBSCT when they went home. Methods: It included establishing a standardized operating protocol to provide bilingual multimedia CDs in health education and self-care, making pamphlets and medical treatment identification cards, building following-up and consulting phone services to support and equip patients' self-care needs. Results: The correct rate of patient's self-care knowledge was improved to 98.9%. Satisfactions of phone-services gained the score of 4.9 in a 5-point scale. The results showed a great improvement in the knowledge of self-care for patients with PBSCT. Conclusions: Teaching the knowledge of PBSCT was complicated. However, applying animation presentation CDs can arise patients' learning motivations and can make it easy to understand. In addition, it is important to provide caring system by keeping close relationship with patients and provide emergency situations in case of accidents. It can also improve patients' knowledge of home care and satisfactions of professional care.