With the Bureau of Health Promotion grant program of Colon Cancer Screening, the purpose of this screening is to achieve early detection and early treatment of colon cancer. According to statistics in 2011, the distribution rate of colon cancer referral sheets was 51.4% and the correct rate of explanation of colon cancer referral sheets was only 30.2%. The related factors of low correct rate included (1) the lack of guidelines of colon cancer referral, (2) no job training program, (3) no evaluation protocol, and (4) inconsistence of teaching contents. By conducting this promotion project, we developed the guidelines of distributing colon cancer referral sheets, on-job training program and teaching content as well as evaluation system. After completing this project, the rate of distributing colon cancer referral sheets was increased to 100%. The correct rate of explaining referral sheets also was raised to 91.9%. These results showed that the project effectively improve the referral process of colon cancer screening. Information of this project may serve as future reference for healthcare providers and nursing professionals in the improvement of colon cancer screening.