Objective: To explore the differences in various physical fitness indicators among physicians and medical staff, nurses and administrative staff, and to under-stand the current status of employees’ health and physical fitness. Methods: This cross-sectional descriptive study utilized convenience sampling, entraining a third-party professional organization to conduct technology-based physical fitness assessments at our hospital. A total of 300 valid participants completed the assess-ments and were evenly divided into three groups based on their occupational catego-ries: physicians and medical personnel (N = 100), nursing staff (N = 100), and ad-ministrative staff (N = 100). The assessment dimensions encompassed body compo-sition indicators (including body mass index BMI and waist-to-hip ratio), muscular strength (handgrip strength), flexibility (sit-and-reach test), and cardiorespiratory endurance. Specifically, cardiorespiratory endurance was evaluated using the three-minute progressive in-place knee-lift step test (3MPKS) , which eliminates the spatial limitations of traditional step-up boxes while offering enhanced safety and precision. For inferential statistics, a one-way analysis of covariance (ANCOVA) was employed, with gender, age, and regular exercise habits controlled as covariates to eliminate demographic confounding factors. Results: After excluding covariate interference, BMI was highest in the administrative group, BMI was highest in the administrative staff group, followed by the nursing staff group and the medical per-sonnel group (p = 0.001, partial η² = 0.042). The effect size approached the moderate range. For sit-and-reach performance, the medical personnel group performed best, followed by the administrative staff group and the nursing staff group (p = 0.020, partial η² = 0.025), with a small-to-moderate effect size. No significant differences were observed in handgrip strength (p = 0.510, partial η² = 0.005), cardiorespiratory endurance (p = 0.770, partial η² = 0.002), or waist-to-hip ratio (p = 0.070) after ad-justment. The effect sizes for these indicators were small. However, the waist-to-hip ratio showed a marginal trend toward statistical significance (p = 0.070, partial η² = 0.018). Norm comparison revealed that men had the most frequent rating of "poor" in flexibility, while both men and women had the most frequent rating of "poor" in cardiorespiratory endurance. Conclusion: This study uses an innovative, simple, and safe method to assess the physical fitness of hospital employees to enhance their willingness to participate, which helps employees manage their own health. After excluding the interference of demographic variables, there are still significant dif-ferences in body composition and flexibility among employees of different occupa-tions. This study can also serve as a reference for medical institutions to promote workplace health promotion strategies. It is recommended to design individualized intervention programs for different occupations and genders to enhance cardi-orespiratory endurance and flexibility, thereby improving overall health and physical fitness performance.