There is a strong connection between character strengths and well-being, research also indicated character strengths improve mental health and stress resistance for college students. However, previous studies suggest that mediators between teenagers’ strengths and well-being can be improved. Research purpose: Investigating the connection between strengths and well-being for Taiwanese teenagers, and to explore the mediating effect of perceived stress. Research method: Survey research was used, a sample of 569 Taiwanese adolescents (in 7th ~9th grade) from different areas was collected. We use the New Inventory of Adolescents’ Strengths (NIAS), well-being scale(TMHC-SF) and the Perceived Stress Scale (PSS) as research tools. After the data were collected, they (correlations, hierarchical regressions, mediations) were inputted and analyzed using the statistical software package SPSS 22. The mediating effect using Hayes (2013) PROCESS bootstrapping to analyze. Results: 1. Significant positive correlation was observed between three dimensions of strengths (Self, Other and Thing) and well-being. It shows significant negative correlation between strengths and perceived stress and significant negative correlation between perceived stress and well-being. 2. Among three dimensions of strengths, “Self” is the only one that will affect well-being indirectly through mediating effect of perceived stress. Conclusion: developing adolescents’ strengths is highly recommended, especially the "Self" strength (including self-respect, optimism and emotion self-awareness) to relieve stress and to eventually bring along well-being.