本論文設計並實現一套應用於電化學感測器之四線式交流阻抗分析儀，其 量測原理採用交流阻抗分析法。系統以 ADuCM355 晶片為核心，透過高速 DAC 產生可調頻正弦波，並經濾波器、單位增益放大器及激勵放大器處理後施加於 待測物。回傳訊號則經轉阻放大器與濾波器調整，再輸入 ADC 進行取樣，並透 過 離散傅立葉變換區塊將時域訊號轉換至頻域。為降低線阻與寄生電容造成的 誤差，本系統採用四線式架構，分別量測電壓與電流。量測結果透過LabVIEW 繪製阻抗圖譜，以利於電路參數之分析。 在系統驗證方面，本研究首先以各元件組成之 Randles 等效電路模型進行 測試，以確認所建構系統之正確性；隨後再以三種不同濃度之赤血鹽搭配雙極 式網版印刷電極進行電化學阻抗量測。實驗結果顯示，本研究所提出之系統能 夠正確量測阻抗特性，並具備可量測極小阻值元件、成本低廉、非破壞性檢測 及攜帶便利等優點。 未來改進方向包括：將轉阻放大器之回授電阻設計為可外接調整並支援自 動切換，擴增交流阻抗分析之量測模式，並整合無線傳輸、液晶顯示與記憶卡 模組，使系統能獨立運作。

This thesis presents the design and implementation of a four-wire electrochemical impedance analyzer for electrochemical sensors. The measurement principle is based on Electrochemical Impedance Spectroscopy . The system is built upon the ADuCM355 platform, where a high-speed DAC generates a frequency-adjustable sine wave that passes through a filter, a unity-gain amplifier, and an excitation amplifier before being applied to the target. The response signal is processed by a transimpedance amplifier and filters, then digitized by an ADC. A Discrete Fourier Transform block is employed to convert the sampled time-domain signals into frequency-domain data. To minimize the effects of lead resistance and parasitic capacitance, the system adopts a four-wire measurement configuration, where voltage and current are measured separately. The results are visualized in LabVIEW to facilitate the extraction and analysis of equivalent circuit parameters. This study verified the accuracy of the proposed system using a Randles equivalent circuit model, and further evaluated its performance through electrochemical impedance measurements with three concentrations of ferricyanide and a bipolar screen-printed electrode. The results show that the system can reliably measure impedance characteristics while providing advantages such as low cost, the capability of measuring very small resistances, non-destructive testing, and portability. Future improvements will focus on making the TIA feedback resistor externally adjustable with automatic switching, expanding the available EIS measurement modes, and integrating wireless communication, an LCD display, and memory card support to enable fully independent system operation.