This study aims to develop an instrument to measure preschool educators’ work passion, as well as establish its reliability and validity. First, through a comprehensive literature review, the instrument of preschool educators’ work passion content was summarized and compiled into a preliminary questionnaire. Second, the instrument was critiqued and reviewed by scholars for establish content validity. Third, the refined instrument was evaluated by using item analysis and exploratory factor analysis from 153 valid responses. The results showed that the questionnaire contained three factors: reaching full potential, job commitment, and job achievement. Confirmatory factor analysis was performed from 152 valid responses. Results showed that the fitness of the model was acceptable, and the instrument had good reliability and validity.