With the expansion of Internet of things (IoT) scale and the extension of its application fields, node energy acquisition has become one of the constraints on the development and application of IoT technology. Wireless energy acquisition is faced with the challenge of how to enhance power conversion efficiency under low input power in the broadband range. This study aimed to improve the capability of energy collection and transmission in wireless communication, an efficient dual-frequency energy harvesting system was proposed, moreover a novel dual-frequency planar inverted-f antenna (PIFA) and its energy conversion circuit for Radio frequency (RF) energy harvesting were designed by studying the characteristics of ambient RF energy and antenna. The developed antenna is designed and manufactured for GSM 900mhz or DCS 1.8 GHz to harvest the ambient energy provided by nearby devices. It is proved that the new dual-frequency antenna still has high efficiency when the input power is less than -20 dbm, and can maximize the receiving energy. The system works with an energy conversion and storage module to convert weak signals into required voltages, the simulation and test results show that the maximum conversion efficiency of the prototype is about 65% at 920MHZ and 1.8GHz at -20 dBm input power.